Description

ProductAvailABIlity:InStock(deliverywithin2-3businessdays,noextrachargefordrop-shipping).

Ordering:ToplaceanorderviacreditcardorPOuseAddtoCartbutton(creatinganaccountisnotrequired).

---

TransfectionReagentfor C6Cells(GliomaCells,CCL-107)

• Twocomponentformulationenhanceslipidmediatedtransfectionefficiency
• Optimizedeasy-to-usetransfectionprotocolprovidedfortransfectionofsiRNA,DNA,mRNA,andmicroRNA
• KitincludesTransfectionEnhancerreagentandrecommendedtransfectionprotocol
• Hightransfectionefficacyinthe presenceofserum
• ExpandyourRNAiapplicationwithareagentoptimizedfordeliveryofbothsiRNAandplasmid
• ReproducIBLetransfectionresults
• Workswellforstandardreversetransfectionandhigh-throughputapplications
• DownloadinvitroC6 transfectionprotocol:[PDF]
• Download C6CRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforC6cellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast94%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsC6TransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

C6 CellLine:

Whileglioblastomaisnotthemostcommontypeofbraincancer,itisthemostaggressiveanddeadliest.Glialcellsareresponsibleforprovidingnourishmentandmechanicalsupporttonervecellsinthebrain.Gliomasaretumorsthatoriginatedinglialcellsandarecharacterizedbasedonlocation.AccordingtotheBrainTumorCenteratJohnsHopkins,malignantgliomascompriseabout70%ofallnewcasesofbraintumorsandtendtorecurdespiteaggressivecombinatorialtreatment.Newtherapiesaredesperatelyneededtoconqueranypotentialchallengesinthetreatmentofthemalignantbraintumorandimprovethesurvivalrateofpatients.Researchatthecellularlevelisahelpfultooltogatherinformationoncellsthatrapidlymetastasizesuchascancerousglialcells.TheC6gliomacelllinewasderivedfromthenervoustissueofarat(Rattusnorvegicus)glialtumorandhasproventobeareliabletransfectionhostforthestudyofbraintumors.ThisstrainofcellsisisolatedbyN-nitrosomethylureabyBendaetal.,exhibitingfibroblastmorphology.Inadditiontobeinganappropriatehostinmammalianbraintumorsstudies,theC6celllineisemployedforpreclinicalresearchworldwideaswellasincellularandmolecularBIOLOGyapplications.C6cellshavebeenknowntoshowglucocorticoidreceptors,andtheyalsomakeforagoodtransfectionresearchmodel.AltogenBiosystemsprovidestwo-componenthigh-efficiencytransfectionreagentkitsfortheC6gliomacellline.

Data:

Figure1.siRNAstargetingLaminA/CmRNAornon-silencingcontrolsiRNAweretransfectedintoC6cellsfollowingtherecommendedprotocol.At48hourspost-transfectionthecellswereanalyzedbyqRT-PCRforLaminA/Cgeneexpressionlevels.18SrRNAlevelswereusedtonormalizetheLaminA/Cdata.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=4).

Figure2.ProteinexpressionofLaminA/CinC6cells.DNAplasmidexpressingLaminA/CorsiRNAtargetingLaminA/CweretransfectedintoC6cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

AltogenBiosystems:
AltogenBiosystemsprovidespre-optimizedtransfectionkitsandelectroporationproductsforlifesciencesandcancerresearch.Transfectionprotocolsareoptimizedforindividualcancercelllines.AltogenBiosystemsdevelopedtwotypesofinvivodeliverykits(animaltransfection):1)Tissue-targetedreagents(deliveryofproteins,DNA,andRNAintoliver,pancreas,orkidneytissues),and2)Broadrangeinvivobiodistributionreagents(PEG-Liposomebasedreagent,Nanoparticle-basedinvivoreagent,Lipid-basedtransfectionkit,andPolymer-basedkit).Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,mRNA,shRNA,siRNA,andothernegativelychargedbiomoleculesinvitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.

AltogenLabsResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#6345)
• 1.5ml(Catalog#6346)
• 1.5mlCRISPR(Catalog#2121)
• 8.0ml(Catalog#7026)

Altogen生命科学公司致力于研发，生产和销售特定细胞系的转染试剂，用于细胞间生物分子的传递，并通过对转染试剂类型的设计将siRNA和质粒DNA有效地转入不同的细胞系和原代细胞内。 Altogen公司开发的聚合物，脂质体，纳米粒子为基础的转染技术分别针对分子生物学，组合化学，和细胞生物学而分别应用。 Altogen定制服务提供符合GLP要求定制研究服务，包括代稳定的细胞系，细胞银行和冷冻保存，焦磷酸测序，克隆，RNA干扰（RNAi）和基因沉默服务，发展分析，siRNA文库筛选，并转染服务。稳定的肿瘤细胞株和原代细胞的产生，可以是非常昂贵和费时。该公司的细胞培养科学家的细胞株的选择，无论是利息或shRNA表达载体的稳定表达的基因改造。标准的RNAi技术服务，包括设计与合成的siRNA的利益，验证siRNA的沉默效率，siRNA转染条件的优化，使高效的基因沉默细胞系或原代培养细胞的靶基因。 转染培养细胞的瞬时或稳定的引入外源性分子和遗传物质（即RNA或DNA），通常是在生物实验室用来研究基因功能，基因表达的调节，生化映射，突变分析，和蛋白质的生产。科学家利用各种载体分子，这种分子，使质粒DNA（PDNA），信使RNA（mRNA），短干扰RNA（siRNA），小分子RNA（miRNA）的，并进入肿瘤细胞株和原代细胞的蛋白质的基因交付。不幸的是，无单提货的方法或转染试剂，可以适用于所有类型的细胞，细胞的细胞毒性和转染效率显着不同，取决于试剂，协议，并正在利用细胞类型。 Altogen生物系统公司提供超过60种类型的细胞的预优化转染试剂盒。 纳米粒子，脂质和聚合物基ALTOGEN ®在体内转染试剂，使交付功能的RNA和DNA分子在体内。 PEG脂质体在体内输送系统减少由于PEG修饰的先天免疫反应，并提供高效的siRNA转染的DNA，并在体内的蛋白质。 由科学“杂志（2010年12月17日）：PEG脂质体在体内转染试剂盒siRNA的特色 Altogen生物系统功能的特定细胞系转染试剂盒：   公司：www.altogen.com