Description

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Transfection Reagent for PANC-1 Cells (Non-endocrine Pancreatic Cancer)

• Proprietary cationic lipids formulation
• High transfection efficiency of small RNA (siRNA, shRNA, miRNA), mRNA, pDNA
• A proven reagent for establishing stable cell lines
• Optimized transfection protocols are adapted for use with both standard & reverse transfection methods
• Download in vitro PANC-1 transfection protocol: [PDF]
• Download PANC-1 CRISPR/Cas9 transfection protocol: [PDF]
• Download PowerPoint presentation for PANC-1 cells transfection kit: [PPT]
• Developed and manufactured by Altogen Biosystems

Transfection Efficiency:

Reagent exhibits at least 85% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.

Transfection Protocol and MSDS:

Download Altogen Biosystems PANC-1 Transfection Protocol: [PDF]

Download MSDS: [PDF]

PANC-1 Cell Line:

According to Nature, pancreatic cancer has a 5-year survival rate of 5%, making the quest for a reliable cure urgent. Pancreatic ductal adenocarcinoma is the fourth primary cause of cancer-related fatalities, comprising nearly 90% of all pancreatic malignancies. PANC-1 is a well characterized and extensively studied cell line that is frequently employed as in vitro model for pancreatic ductal adenocarcinoma research. The PANC-1 cell line plays an essential role in pancreatic cancer therapeutic assays. The PANC-1 cell line was established from pancreatic duct tissue of a 56-year-old Caucasian male with epithelioid carcinoma. PANC is short for pancreas, referring to the Human pancreas, where cancer can be found. Also, the PANC-1 cell line serves as a suitable transfection host. The growth of these cells is inhibited by L-asparaginase.

PANC-1 cells are used as an in vitro model of non-endocrine pancreatic cancer for tumorigenicity studies. These cells possess the type B phenotype for glucose-6-phosphate dehydrogenase G6PD and overexpress heregulin/human epidermal growth factor receptor 2 (HER2/neu) oncogene, which is present in 60 to 70% of human pancreatic carcinomas but are estrogen receptor (ER) negative. PANC cells have been shown to be negative for MUC4 and positive for Smad4, a TGF beta signaling cascade protein inactivated in human gastrointestinal cancers. PANC can be induced to differentiate into hormone-producing islet-like clusters following stimulation by the FGF2 growth factor. The PANC-1 cell line has an estimated doubling time of 52 hours with a modal chromosome number of 63. Also, these cells have Glucose-6-phosphate dehydrogenase activity of the slow mobility of B type. Altogen Biosystems provides proprietary cationic liposome formulation that demonstrate high efficacy transfection of PANC-1 cells. Transfection kit is preoptimized for the intracellular delivery of plasmid DNA, siRNA, and mRNA molecules into PANC-1 non-endocrine pancreatic cancer cell line.

Data:

Figure 1. Cyclophilin B silencing efficiency was determined by qRT-PCR in the PANC-1 cells transfected by Cyclophilin B siRNA or non-silencing siRNA control following the recommended transfection protocol. Cyclophilin mRNA expression levels were measured 48 hours post-transfection. 18S rRNA levels were used to normalize the Cyclophilin B data. Values are normalized to untreated sample. Data are presented as means ± SD (n=3).

Figure 2. Protein expression of Cyclophilin B in PANC-1 cells. DNA plasmid expressing Cyclophilin B or siRNA targeting Cyclophilin B were transfected into PANC-1 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

Altogen Biosystems:
Altogen Biosystems provides preoptimized transfection products for life science research applications. Transfection protocols are optimized for individual cancer cell lines. Altogen Biosystems developed two types of in vivo delivery kits for animal research: Tissue-targeted reagents (delivery to liver, pancreas, and kidney tissues), and broad range in vivo delivery reagents (PEG-Liposome, Nanoparticle-based, Lipid-based, and Polymer-based kits). Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of proteins, DNA, RNA, and any other negatively charged molecules in vitro and in vivo. Read more about transfection technology at Altogen’s Transfection Resource.

Altogen Labs Research Services:

Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).

Volume Options:

• 0.5 ml (Catalog #3225)
• 1.5 ml (Catalog #3226)
• 1.5 ml CRISPR (Catalog #2187)
• 8.0 ml (Catalog #7076)

Altogen生命科学公司致力于研发，生产和销售特定细胞系的转染试剂，用于细胞间生物分子的传递，并通过对转染试剂类型的设计将siRNA和质粒DNA有效地转入不同的细胞系和原代细胞内。 Altogen公司开发的聚合物，脂质体，纳米粒子为基础的转染技术分别针对分子生物学，组合化学，和细胞生物学而分别应用。 Altogen定制服务提供符合GLP要求定制研究服务，包括代稳定的细胞系，细胞银行和冷冻保存，焦磷酸测序，克隆，RNA干扰（RNAi）和基因沉默服务，发展分析，siRNA文库筛选，并转染服务。稳定的肿瘤细胞株和原代细胞的产生，可以是非常昂贵和费时。该公司的细胞培养科学家的细胞株的选择，无论是利息或shRNA表达载体的稳定表达的基因改造。标准的RNAi技术服务，包括设计与合成的siRNA的利益，验证siRNA的沉默效率，siRNA转染条件的优化，使高效的基因沉默细胞系或原代培养细胞的靶基因。 转染培养细胞的瞬时或稳定的引入外源性分子和遗传物质（即RNA或DNA），通常是在生物实验室用来研究基因功能，基因表达的调节，生化映射，突变分析，和蛋白质的生产。科学家利用各种载体分子，这种分子，使质粒DNA（PDNA），信使RNA（mRNA），短干扰RNA（siRNA），小分子RNA（miRNA）的，并进入肿瘤细胞株和原代细胞的蛋白质的基因交付。不幸的是，无单提货的方法或转染试剂，可以适用于所有类型的细胞，细胞的细胞毒性和转染效率显着不同，取决于试剂，协议，并正在利用细胞类型。 Altogen生物系统公司提供超过60种类型的细胞的预优化转染试剂盒。 纳米粒子，脂质和聚合物基ALTOGEN ®在体内转染试剂，使交付功能的RNA和DNA分子在体内。 PEG脂质体在体内输送系统减少由于PEG修饰的先天免疫反应，并提供高效的siRNA转染的DNA，并在体内的蛋白质。 由科学“杂志（2010年12月17日）：PEG脂质体在体内转染试剂盒siRNA的特色 Altogen生物系统功能的特定细胞系转染试剂盒：   公司：www.altogen.com