Description:	The Cell-Check™ Viability/Cytotoxicity Kit for Animal Cells provides a two-color fluorescence staining on both live and dead cells using two probes that measure two recognized parameters of cell viability — intracellular esterase activity and plasma membrane integrity. The kit is suitable for use with fluorescence microscopes, fluorescence multiwell plate scanners and flow cytometers and other fluorescence detection systems. The assay principles are general and applicable to most eukaryotic cell types, including adherent cells and certain tissues, but not to bacteria or yeast. It is generally faster, less expensive, safer and a more sensitive indicator of cytotoxic events than alternative methods. Live cells are distinguished by the presence of ubiquitous intracellular esterase activity, determined by the enzymatic conversion of the virtually nonfluorescent cell-permeant calcein AM to the intensely fluorescent calcein. The polyanionic dye calcein is well retained within live cells, producing an intense uniform green fluorescence in live cells (Ex/Em ~495 nm/~520 nm). Propidium iodide (PI) enters cells with damaged membranes and undergoes a 30-fold enhancement of fluorescence upon binding to nucleic acids, thereby producing a bright red fluorescence in dead cells (Ex/Em ~528 nm/~617 nm). PI is excluded by the intact plasma membrane of live cells.
Order #:	A017-ABP
Unit Size:	1000 assays
Supplier:	ABP Biosciences
Shipping:	RT
Storage:	−20 ℃
Subcategory:	Cell Viability
More information:	Go to webpage

   苏州蚂蚁淘生物技术有限责任公司是一家2001成立的经营生命科学用品的高科技企业，除了代理国际*公司生产的试剂、仪器、设备和耗材等产品外，也把寻找新的肿瘤标志物定位为公司的长远发展方向。 代理和经销的产品分为两类：医学类产品和生物类产品。医学类产品主要包括：肿瘤早期诊断试剂（意大利Xeptagen），甲状腺类检测产品（德国Medipan），过敏原检测系统（美国Hitachi），载玻片离心机及染色仪（德国Tharmac），高强聚焦超声刀（中国 希复康）。生物类产品主要包括：免疫学和神经生物学产品（以色列Alomone），细胞因子（以色列Prospec），生物化学和分子生物学类产品（美国Qbiogene），酶免分析试剂盒（美国Cytimmune），核酸纯化类产品（美国Omega），生物化学和免疫类产品（美国Alexis），酶免分析试剂盒和各种抗体（美国Cytimmune），多种抗原和抗体（美国Fitzgerald），细胞和分子生物学产品（美国Cambrex）等30多种。   order# description amount BMEG02 Bacillus megaterium protoplasts ready for transformation (strain WH320;1) Material is sufficient for 4 transformations plus control experiment. 5 x 500 µl BMEG50 Bacillus megaterium protoplasts, strain MS941 5 x 500 µl BMEG31 pC-His1623hp shuttle vector, C-term. 6xHis-tag; lyophilized DNA 10 μg BMEG32 pN-His-TEV1623hp shuttle vector, N-term. His-tag,TEV-site 10 μg BMEG30 p3STOP1623hp, shuttle vector, lyophilized DNA 10 μg BMEG33 pSPLipA-hp, shuttle vector with signal sequence, lypophilized DNA 10 μg BMEG34 pSPYocH-hp, shuttle vector with signal sequence, lypophilized DNA 10 µg BMEG03 pWH1520 shuttle vector, original; lyophilized DNA 5 µg BMEG10 pMM1522 shuttle vector, improved; lyophilized DNA 10 µg BMEG11 pMM1525 shuttle vector with signal sequence; lyophilized DNA 10 µg BMEG12 pHIS1522 shuttle vector, 6xHis-tagged; lyophilized DNA precursor of BMEG20 10 µg BMEG13 pHIS1525 shuttle vector with signal sequence; 6xHis-tagged; lyophilized DNA 10 µg BMEG14 pSTREP1525 shuttle vector with signal sequence; Strep-tagged; lyophilized DNA 10 µg BMEG15 pSTREPHIS1525 shuttle vector with signal sequence; Strep/6xHis double-tagged; lyophilized DNA 10 µg BMEG20 pC-His1622 shuttle vector, C-term. 6xHis-tag; lyophilized DNA 10 µg BMEG21 pC-Strep1622 shuttle vector, C-term. Strep-tag; lyophilized DNA 10 µg BMEG22 pN-His-TEV1622 shuttle vector, N-term. His-tag,TEV-site 10 µg BMEG23 pN-Strep-TEV1622 shuttle vector, N-term. Strep-tag,TEV-site 10 µg BMEG24 pN-StrepXa1622 shuttle vector, N-term. Strep-tag; Xa site; lyophilized DNA 10 µg BMEG25 pSTOP1622, shuttle vector, lyophilized DNA 10 µg BMEGT702 Bacillus megaterium protoplasts, strain MS941, pretransformed with pT7-RNAP 5 x 500µL BMEGT701 Bacillus megaterium high yield T7 gene expression kit,  includes pretransformed protoplasts BMEGT702 (5 x 500µl), pPT7 cloning vector and pPT7-GFP control vector (vectors lyophilized, 10 µg each) Kit BMEGT710 Bacillus megaterium pPT7 cloning vector, lyophilized 10 µg shipped at RT, protoplasts shipped on dry ice  store lyophilized vectors at 4°C, reconstituted vectors at -20°C, protoplasts at -70°C All vectors are E. coli / B. megaterium shuttle vectors Please note that protoplasts of Bacillus megaterium have a limited shelf life of some months only, depending on the date of production. Therefore purchase and use of protoplasts must be thoroughly planned.