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Protein Extraction Kit is designed for quick and effective preparation and isolation of total proteins. The kit contains optimized reagents and materials that efficiently extract and isolate proteins from any cells and tissues. Lysis Beads in the kit enable efficient and rapid lysis of cells and tissues to allow complete release of proteins. Extraction Buffer is pH optimized to maximize protein yield. The total proteins isolated by this kit can be used for a variety of applications, including protein microarray assays, Western blots/immunoprecipitation, gel mobility shift assays, and other procedures.
- Effectively lyse cells and tissues, producing non-denatured protein extract
- Metal lysis beads provide efficient release of proteins to maximize yield
- Ready to use
- Quick and easy protocol
- Replaces the extraction buffer and lysis beads provided in Antibody Array Assay Kit
PRODUCT DETAILS
- – Size: 20 reactions (Extraction Buffer: 10 mL; Lysis Beads: 20 tubes)
- – Storage Condition: 4°C
- – Shelf-life: 1 year
- – User’s Guide: Protein Extraction Kit User’s Guide
- – Material Safety Data Sheet (MSDS)
抗体芯片种类: 抗体芯片特点: 50uL的液质样本、200ug的总蛋白量即可完成多指标检测; 适用于血清/浆、培养上清及细胞、组织裂解液等多种生物样本; 可以有效避免采用传统方法所引入的批次间实验误差; 扩大单个样本的信息量并可做不同分析物间的交互分析。 抗体芯片原理: 抗体芯片文献: Izzotti A, et al. Relationships between pulmonary microRNA and proteome profiles, systemic cytogenetic damage and lung tumors in cigarette smoke-exposed mice treated with chemopreventive agents. Carcinogenesis, 2013, 34(10): 2322-2329. Straussman R, et al. Tumour microenvironment elicits innate resistance to RAF inhibitors through HGF secretion. Nature, 2012, 487(7408): 500-504. Bagnis A, et al. Aqueous humor oxidative stress proteomic levels in primary open angle glaucoma. Exp Eye Res, 2012, 103: 55-62. Moon K M, et al. The effect of secretory factors of adipose-derived stem cells on human keratinocytes. Int J Mol Sci, 2012, 13(1): 1239-1257. Saccà S C, et al. New proteins as vascular biomarkers in primary open angle glaucomatous aqueous humor. Invest Ophth Vis Sci, 2012, 53(7): 4242-4253. Yalcin A, et al. Nuclear targeting of 6-phosphofructo-2-kinase (PFKFB3) increases proliferation via cyclindependent kinases. J Bio Chem, 2009, 284(36): 24223-24232. Tang Y L, et al. Hypoxic preconditioning enhances the benefit of cardiac progenitor cell therapy for treatment of myocardial infarction by inducing CXCR4 expression. Circ Res, 2009, 104(10): 1209-1216.


