Put more eggs in your basket! CARD HyperOva® (Japanese Patent No. 5927588) is an ultra-superovulation reagent that induces ovulation at higher efficiency when compared with the conventional superovulation induction method (PMSG-hCG method). Following the conventional method, we were only able to obtain approximately 20 ova per mouse (C57BL/6J) on average; using CARD HyperOva®, however, we can obtain as many as 3-4 times more ova. Protocol:1. Administer CARD HyperOva(0.2mL/head; i.p) to Female mice (C57BL/6 strain, 4 weeks of age) 2. At 48h after the administration of HyperOva, administer 7.5IU hCG(i.p.) (not included).3. At 17h after hCG injection, sacrifice mice by cervical dislocation, and collect their oviducts and transfer them to a fertilization dish.