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蚂蚁淘在线 / 品牌中心 / Alstembio / Alstembio/Celloticityasayasaykit/CA01/500试验

Alstembio/Celloticityasayasaykit/CA01/500试验

价格
￥4200.00
货号：CA01
浏览量：127
品牌：Alstembio
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商品描述
 

EZQuant™CytotoxicityAssayKit:#CA01

 

ProductDescription

TheEZQuant™CytotoxicityAssayKitisusedtoassesscelldeath.ItmeasuresthelevelofLDHreleasedwhencellsundergostressorinjuryfromchemicalsthataretoxictothecells.ThereagentisreducedbyNADHthatwasoxidizedbytheextracellularLDH.Therefore,theamountofformazanproducedisdirectlyproportionaltothenumberofdeadcells.

PairwithEZQuant™CellQuantifyingKit

TheEZQuant™CellQuantifyingKitconvenientlydeterminesthenumberofviablecellsincellproliferationandcytotoxicityassays.Thisproductisusefulfordrugscreeningandcytotoxicitytestingofchemicals.Meanwhile,theEZQuant™CytotoxicityAssayKitdeterminesthenumberofdamagedcellsandthedegreeofcytotoxicity.Combiningthesetwoproductswillallowresearcherstofullyunderstandthecellhealthconditionsundervariouscytotoxicenvironments.

Features

  • Highlinearityrange
  • Longshelf-lifewithoutdegradation(2monthsat0-5°C)
  • Simplehomogenousassayornon-homogenous(multiplex)assaypossIBLe

ClicktoseeourRESULTS.

 
ProductSpecifications
ProductNameCytotoxicityAssayKit
Catalog#CA01
Size500tests(CA01)/2000tests(CA04)
ShippingRoomTemperature
StorageandStABIlityStoreat0-5°C
QualityControlAppearance,blank,andsensitivitytestsareperformedforeachlot.Onlylotsthatpassthefollowingcriteriaareoffered:BlankAbsorbance(460nm)≤0.700SensitivityAbsorbance(460nm)≥1.000
RestrictedUseForResearchUseOnly.Notforuseindiagnosticortherapeuticprocedures.
ProtocolDownload
 
  • Homogenousassay
  • Non-homogenousassay
CellConcentrationOptimization
  1. Collectcellsandwashthemwiththeassaymedium.PreparecellsUSPensionto5×105cells/mlintheassaymedium.
  2. Add100μloftheassaymediumtoeachwellofaflat-bottom96-welltissuecultureplate.
  3. Prepare2-foldserialdilutionofeachwellintriplicatesetofwellsforthehigh-control,low-controlandbackgroundcontrol(mediumonly).Serialdilution:Addthecellsuspension(5×105cells/ml)tothefirstwellandmixbypipetting.Thiswellcontainsthemaximumnumberofcells(2.5×104cells/well).Transfer100μlfromthefirstwelltothenextwell,andmixbypipetting.Repeatthisprocedure.
  4. Incubatetheplateat37°CforanappropriatetimeinaCO2incubator.Usethesameincubationtimeinthecytotoxicityassay.
  5. Add10μlofthelysisbuffertoeachwellofthehighcontrol.
  6. Incubatetheplateat37°Cfor30minutesinaCO2incubator.
  7. Add100μloftheworkingsolutiontoeachwell.Protecttheplatefromlightandincubateitattheroomtemperaturefor30minutes.
  8. Add50μlofthestopsolutiontoeachwell.
  9. Measuretheabsorbanceat490nmbyamicroplatereader.
CytotoxicityAssay
  1. Add50μlofcellsuspensiontoeachwellofaflat-bottom96-welltissuecultureplate.Foradherentcells:incubatetheplateat37°CovernightinaCO2incubatortoallowthecellstoadhereandthenreplacetheassaymediumwith50μloffreshassaymedium.
  2. Add50μlofassaymediumcontainingtestsubstancethatadjustedtothedesiredconcentration.
     BackgroundcontrolTestsubstanceLowcontrolHighcontrol
    Assaymedium100μlN/A50μl50μl
    CellsuspensionN/A50μl50μl50μl
    TestsubstanceinculturemediumN/A50μlN/AN/A
    LysisbufferN/AN/AN/A10μl
  3. Incubatetheplateat37°CforanappropriatetimeperiodinaCO2incubator.
  4. Add10μlofthelysisbuffertoeachwellofthehighcontrol.Incubatetheplateat37°Cfor30minutesinaCO2incubator.
  5. Add100μloftheworkingsolutiontoeachwell.Protecttheplatefromlightandincubateitattheroomtemperaturefor30minutes.
  6. Add50μlofthestopsolutiontoeachwell.
  7. Measuretheabsorbanceat490nmbyamicroplatereader.
CalculateCytotoxicity

Calculatetheaverageabsorbancefromeachtriplicatesetofwellsandsubtractthebackgroundcontrolvaluefromeachabsorbanceone.Calculatethepercentcytotoxicitybythefollowingequation:Cytotoxicity(%)=(Testsubstance-Lowcontrol)/(Highcontrol-Lowcontrol)x100

Alstembio定制病毒包装慢病毒包装请求（Web表单） 逆转录病毒打包请求（Web表单）我们提供快速的慢病毒和逆转录病毒包装服务，以使用您的病毒构建体生产高质量，高滴度的病毒颗粒，周转时间为1-2周。节省您自己准备病毒的时间和精力，并获得随时可以转导的病毒颗粒。我们的包装服务的特点：周转时间： 10个工作日或更少质量：符合国家卫生研究院《生物安全2级》标准的一流BSL-2设备中的病毒生产，并具有严格的质量控制滴度测量：通过qPCR确定的准确病毒滴度，以测量每毫升感染单位（IFU / ml）所需材料：如果您打算准备自己的DNA，我们要求您提供10-400 µg DNA，具体取决于所需的滴度和体积可交付成果：对于100 µl的体积，我们提供5 x 20 µl的病毒颗粒等分试样，对于200 µl的体积，我们提供10 x 20 µl的等分试样。也可以使用其他配置。