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蚂蚁淘在线 / 品牌中心 / Alstembio / Alstembio/RetrovirusiPCreProgrammingKit/RF101

Alstembio/RetrovirusiPCreProgrammingKit/RF101

价格
￥19000.00
货号：RF101
浏览量：127
品牌：Alstembio
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商品描述

RetrovirusiPSCReprogrammingKit:#RF101

ProductDescription

Humaninducedpluripotentstemcells(iPSCs)canbederivedfromsomaticcellsthroughareprogrammingprocessdrivenbyectopicexpressionofadefinedsetofreprogrammingfactors:Oct4,Sox2,Klf4andc-Myc.ThesehiPSCssharethepropertiesofself-renewalandpluripotencywithhumanEScells,andcanthereforebeusedasarenewablesourceforalldifferentiatedcelltypesofthebody.HumaniPSCscanbegeneratedfrompatientsofvirtuallyanygeneticbackground.Retrovirusesareefficienttoolsfordeliveringheritablegenesintothegenomeofdividingcells.TheVSV-Gpseudotypedretrovirushasawiderangeoftargetsincludingbothmammalianandnon-mammaliancells,andareusuallysilencedinEScells.TheretrovirusiscommonlyusedingeneratingiPSCsbecauseofitshighreprogrammingefficiency.TheHumaniPSCellReprogrammingRetrovirusKitofferssuchopportunitytogenerateiPSCsfromvarioustissuesandcelltypes.
ProductSpecifications
ProductNameRetrovirusiPSCReprogrammingKit
Catalog#RF101
Content

Retroviruscocktailcontaining:humanOct4retrovirus(cat#RF01O)2vials,20μleachhumanSox2retrovirus(cat#RF01S)2vials,20μleachhumanKlf4retrovirus(cat#RF01K)2vials,20μleachhumanc-Mycretrovirus(cat#RF01M)2vials,20μleachGFPretrovirus(cat#RF01G)1vial,20μleachTransPlusvirustransductionenhancer(cat#V020)1vial,100μl

ShippingDryIce
StorageandStABIlityStoreat-80°C.Thisproductisstableupto6monthswhenstoredasdirected.
QualityControlEachlotofHumaniPSCellReprogrammingRetrovirusKitistestedforsterilityandsuccessfulreprogramming.
RestrictedUseForResearchUseOnly.Notforuseindiagnosticortherapeuticprocedures.
ProtocolDownload
 
  • Overview
  • Procedure

Humaninducedpluripotentstemcells(iPSCs)canbederivedfromsomaticcellsthroughareprogrammingprocessdrivenbyectopicexpressionofadefinedsetofreprogrammingfactors:Oct4,Sox2,Klf4andc-Myc.ThesehiPSCssharethepropertiesofself-renewalandpluripotencywithhumanEScells,andcanthereforebeusedasarenewablesourceforalldifferentiatedcelltypesofthebody.HumaniPSCscanbegeneratedfrompatientsofvirtuallyanygeneticbackground.

Retrovirusesareefficienttoolsfordeliveringheritablegenesintothegenomeofdividingcells.TheVSV-Gpseudotypedretrovirushasawiderangeoftargetsincludingbothmammalianandnon-mammaliancells,andisusuallysilencedinEScells.TheretrovirusiscommonlyusedingeneratingiPSCsbecauseofitshighreprogrammingefficiency.TheHumaniPSCellReprogrammingRetrovirusKitofferssuchopportunitytogenerateiPSCsfromvarioustissuesandcelltypes.

Materialsyouwillneed:

HumanESmedium

DMEM/F12Invitrogen10565-042  
Pen/strepInvitrogen15140-1221X1mL
L-GlutamaxInvitrogen35050-0612mM2mL
nonessentialaminoacidsInvitrogen11140-0500.1mM2mL
2-mercaptoethanolSigmaM75220.1mM(1000x)0.2mL
knockoutserumreplacementInvitrogen10828-02820%40mL
bFGFStemRDbFGF-05010ng/mL0.2mL
Total200mL
 
Alstembio定制病毒包装慢病毒包装请求（Web表单） 逆转录病毒打包请求（Web表单）我们提供快速的慢病毒和逆转录病毒包装服务，以使用您的病毒构建体生产高质量，高滴度的病毒颗粒，周转时间为1-2周。节省您自己准备病毒的时间和精力，并获得随时可以转导的病毒颗粒。我们的包装服务的特点：周转时间： 10个工作日或更少质量：符合国家卫生研究院《生物安全2级》标准的一流BSL-2设备中的病毒生产，并具有严格的质量控制滴度测量：通过qPCR确定的准确病毒滴度，以测量每毫升感染单位（IFU / ml）所需材料：如果您打算准备自己的DNA，我们要求您提供10-400 µg DNA，具体取决于所需的滴度和体积可交付成果：对于100 µl的体积，我们提供5 x 20 µl的病毒颗粒等分试样，对于200 µl的体积，我们提供10 x 20 µl的等分试样。也可以使用其他配置。