AlbuVoid™Selectively Voids Albumin, Binds Low Abundance Proteome Albumin voids in flow through, 95% 30 minute protocol Low abundance enrichment equivalent or better than hexapeptides or antibodies On-bead digestion protocols, efficient LC-MS workflows Disposable, cost-effective, no column regeneration or cross-contamination Mild elution maintains native structure with retained enzymatic, functional & bioactivities Species agnostic View Details 2DE analysis of AlbuVoid™ treated sheep serum. Samples were reduced, alkylated and total protein normalized. The circled regions indicate the albumin zone. 1: Control. 2: AlbuVoid™ Eluate. Bottom: AlbuVoid™ Eluate silver stain. AlbuSorb™Selectively Binds Albumin Removes serum albumin 90% Economical small ligand surface architecture (not dye-based), bio-affinity performance Consumable, cost-effective, no column regeneration or cross-contamination Species agnostic Compatible with: LC-MS Microarrays Functional assays Non-reduced SDSPAGE profiles Left lane: Normal human pooled serum control Right lane: Flow-through from AlbuSorb™ AlbuVoid™ LC-MS On-BeadSelectively Voids Albumin, Binds Low Abundance Proteome before LC-MS use Seamless workflows Label, label free & glyco- compatible Unique proteolytic efficiencies View Details Total LC-MS/MS Proteins identified at 2 digestion times: 4 hours (4h) & overnight (O/N), single 3 hour LC gradient. AlbuVoid™ PLUSAlbumin and IgG Depletion From Serum/Plasma for Proteomics IgG removal 90% (70-80% of total Immunoglobulins removed) Albumin removal 95% Seamless and simple 1 hour protocol Low abundance enrichment equivalent to immuno-affinity Disposable, cost-effective, no column regeneration or cross-contamination Works for most species tested including human, sheep, rat, mouse, bovine On-bead protocols improve workflow and efficiency, especially suited to targeted proteomics Suitable for LC-MS, 1 and 2D Gels, ELISAs, Enzyme and other Functional Assays. View Details 20µg of protein loaded on to 10% tris bis gel A - Serum alone – shows IgG and Albumin regions B - NuGel™ ProteinA treated serum – shows IgG depletion C - AlbuVoid™ treated serum – shows Albumin depletion D - AlbuVoid™ PLUS treated serum – shows IgG and Albumin depletion AlbuSorb™ PLUSSelectively Binds Albumin & Immunoglobulin 400 μg total serum protein mass per prep 85% Albumin, 85% IgG depleted from 25 μl serum View Details AlbuSorb™ combines with an optimized immobilized Protein A to create AlbuSorb™ PLUS. Unlike immuno-affinity, the surfaces utilized are disposable eliminating cycle to cycle variance and crosscontamination. Lane 1: Human Serum Control Lane 2: Serum after treatment with AlbuSorb™ PLUS Cost-effective, not derived from biologicals No specialized instruments or HPLC Economical surface chemistries, not derived from biologicals No regeneration, so no prep to prep variability Simple, fast microfuge bind/wash/elute protocolsOn-Bead DigestionEfficient workflows, quality LC-MS/MS data Simple, reproducible workflows Equivalent or better than in-solution digestion Seamless to LC-MS, no desalting or C18 separations Unique proteolytic efficienciesEnrichment/DepletionDiverse strategies, species agnostic Products support strategies for both enrichment of low abundance proteomes, or depletion of high abundance proteins Species agnostic not derived from biologicalsFunctional IntegrityMaintained throughout all separations Mild buffer conditions maintains native structure with retained enzymatic, functional & bio-activities Supports enzyme biomarker assays Functional & Chemical Proteomics Structural & activity-probe Proteomics Top-down & ArrayBridge PEP ProteomicsRaise Your Research

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