KinaSorb™ Kinase (& ATP binding proteins) Enrichment Reagent

• Non-covalentimmobilization of phosphate group with optimal nucleotide orientation &specificity
• Enrichment3-5X Kinase enrichment, protein recoverable, ~200 µg
• 60minute, scaleable protocol compatible with functional assays, electrophoresisand LC-MS
• Phosphataseactivity & cyclic nucleotide phosphodiesterase (PDE) activity notdetectable
• Improvesprotein normalization when comparing heterogeneous tissues
• On-beaddigestion protocols for LC-MS annotation

KinaSorb™ is a new reagent kit used for the enrichment and isolation of kinases. ATP – the common substrate for kinases, is reversiblyimmobilized with optimal orientation and specificity, to metallic oxide particles, producing a single-use highlyefficient enrichment method for kinase & other ATP binding proteins. The standard prep protocol starts with 100 µl of clarified cellular extracts, or approximately 2 mg total cellular extract protein, but the process can be scaled up or down to accommodate different sample volumes and protein concentrations. The kit includes all necessary reagents for immediate use.

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