Polymer Coated Silica Affinity Matrices For Glycoprotein Enrichment

• Non-specific sites are virtually eliminated by a polymer coating
• Stable across a wide pH range 4 – 10
• 1000Å, 50µm suitable for LC and batch processes
• Cis-diol specific, enriches heterogeneous sets of glycoproteins
• Unmasks glycoproteins from high abundance proteins, most notably albumin
• Efficient new surface technology ideal for proteomic applications
• Enriches glycoproteins from blood, serum, plasma, tissue or cell culture media.
• Sorbitol elution at pH 7.5 – 8.5
• Adaptable to liquid chromatography (LC) and high-throughput 96-well format

NuGel™ Phenyl Boronic Acid is a derivative of NuGel™ polyepoxy silica affinity support. This affinity support contains phenyl boronic groups at the end of hydrophilic spacer arms and is used to bind ligands containing cis-diols, glycoproteins.

NuGel™ Phenyl Boronic Acid Technical Data

Characteristics Of The Matrix
Spacer Arm	Polymerized hydrophilic carbon chain
Porosity	1000Å
Average Particle Size	50um

Special Features:

• Binds ligands containing 1,2 cis-diol groups of glycoproteins.
• pH stable from 4 to 10.

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