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Purifieswhole infectious non-enveloped virus & non-infectious envelopedvirus
Preparesviral samples for subsequent detection and analysis
Alternativeor complementary to ultracentrifugation
“…lovethis vs sucrose cushions!” MaggieL. Bartlett, BS PhD, JohnsHopkins Bloomberg School of Public Health
orcan complement ultracentrifugation, for viral proteomic enrichment350X (Fragnoudet al, 2016 http://www.ncbi.nlm.nih.gov/pubmed/26572220)
Isolatesantigenic virions, enveloped and non-enveloped
Enrichesfor viral nucleic acids; detergent, chaotrope, Trizol® compatible
Viraffinity™is a unique water-insoluble elastomeric polyelectrolyte that has beenengineered for the capture and recovery of viruses. It is supplied asa suspension reagent ready for use. Applications include:purification of whole infectious non-enveloped virus, virions, viralcomponents, and sample preparation for subsequent detection andanalysis.
Viraffinity™is directly added to a pre-conditioned sample, is then mixed andcentrifuged using standard micro-centrifuges (not ultra). Thecentrifuged pellet contains polyelectrolyte-bound viruses that canthen be recovered using a moderately alkaline pH solution.
RelatedViraffinity™ Kits
ViraPrep™Mammalcontains Viraffinity™and all necessary buffers and protocols for mammalian virus andvirion isolation.
https://www.biotechsupportgroup.com/ViraPrep-Mammal-p/vpm.htm
ViraPrep™Lambdais a complete application kit containing Viraffinity™and all the necessary reagents for obtaining high purity DNA fromplate or liquid lysates, suitable for amplification, automatedfluorescent sequencing and other common molecular biology techniques.
https://www.biotechsupportgroup.com/ViraPrep-Lambda-p/vlk.htm
