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hCollagenIV | |
| Manufacturer/Trade: | Exocell/CollagenIVH |
| CatalogueNumbers: | 1015StripPlate |
| Methodology: | CompetitiveELISA |
| Summaryofprocedure: | CollagenIVHisanindirectcompetitiveELISAdesignedtomeasuretypeIVcollageninBIOLOGicalandtissueculturespecimensofhumanorigin.Theassaymaybecompletedina“normalsensitivity”mode,withausefulassayrangeof0.156-10ugcollagenIV/mL.Aslightlydifferentprocedure,the“enhancedsensitivity”modetypicallyyieldsausefulassayrangeof0.02-2.5ug/mL,andissensitiveenoughtodetectcollagenIVinurinespecimens. Tocompletetheassay,sampleandgoatanti-collagenIVantibodyareaddedtohumancollagenIVcoatedwells.TheantibodyinteractsandbindswiththecollagenIVimmobilizedtothestationaryphaseorwiththeantigeninthefluidphase,hencethenotionofcompetitivebinding.Afterasuitableincubationperiod,theplatesarewashed,andarabbitanti-goat-HRPconjugateisusedtodetectboundantibody.Afterwashing,onlytheantibody-conjugateboundtothestationaryphaseremainsinthewell,andthisisdetectedusingachromogenicreaction.ColorintensityisinverselyproportionaltothelogarithmofcollagenIVinthefluidphase.Theassaymaybecompletedinlessthan3hours. The“enhancedsensitivity”modeiscompletedbyplacingthesamplesandanti-collagenIVantibodyintotubesandincubating2-24hours.Here,theantibodycanonlyreactwiththecollagenIVofthefluidphase.Subsequently,aliquotsofthemixtureareaddedtotheplatewhereremainingfreeantibodycanreactwiththeantigenofthestationaryphase.Theboundantibodyisdetectedasdescribedabove. |
| SpecimenRequired: | 50-100uLCellCultureExtract,Urine |
| AssayRange: | NormalSensitivity:0.156-10ug/mL EnhancedSensitivity:0.02-2.5ug/mL |
| Precision: | IntraassayandinterassayprecisionforsampleswithintheusefulrangeoftheassayhaveaC.V.<10%ofthemean. |
 ©2012ExocellInc. | |
