Exocell/m Albumin/1011 Strip Plate

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货号:1011StripPlate
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品牌:Exocell
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商品描述
Exocell的EIA稀释剂是一种专门配制的稳定剂,用于稳定Ethos的Nephrin ELISA试剂盒中的样品。如果预期样品会进一步稀释,例如血清所需,建议使用其他稀释剂进行测定。
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mAlbumin


Manufacturer/Trade:Exocell/AlbuwellM
CatalogueNumbers:1011StripPlate
Methodology:CompetitiveELISA
Summaryofprocedure:AlbuwellMisadirectcompetitiveELISA(antibodycapture)designedtomonitorkidneyfunctioninthemousebymeasuringurinaryalbumin.Tocompletetheassay,sampleandanti-mousealbuminAb-HRPconjugateareaddedtoalbumincoatedwells.Theantibodyconjugatereactswitheitherthealbumincoatedtotheplateorwiththatinthefluidphase,hencethenotionofcompetitivebinding.

Aftera30minuteincubation,theplateiswashedtoremovereactantsinthefluidphase.Onlyconjugatethatwasboundtothealbuminontheplateremains,anditisdetectedusingTMBinachromogenicreaction.Colorintensityisinverselyproportionaltothelogarithmofthealbuminconcentrationinthefluidphase.Theassaymaybecompletedinlessthananhour.

SpecimenRequired:Urine,10ul
AssayRange:0.156-10ug/mL
Precision:IntrassayandinterassayprecisionforsampleswithinusefulrangeoftheassayhaveaC.V.≤10%ofthemean.



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