NeuroVue^® Dye Filter is useful for neuronal tract tracing studies of up to 3-4 weeks. Spectrally compatible with eGFP and YFP in many systems, and NeuroVue^® Maroon, & Jade. Spectral unmixing required for use with NeuroVue^® Red.

1cm² filter coated with the lipophilic reddish orange emitting dye, NeuroVue^® Orange

(ex max=550nm; em max=570nm).

Advantages:

• Convenient, ready-to-use coated filter format
• More precise control of dye insertion point
• No messy oils, pastes or hard-to-position crystals
• Diffusion properties comparable to or better than other commercially available neurotracing dyes
• More focal results (e.g. labeling of small sets of axons within a pathway)
• Available in multiple colors, including far red, for multi-tract tracing and improved results even in tissues with high myelin expression

Recent Publications:

Diffusion and Imaging Properties of three new lipophilic tracers, NeuroVue^® Maroon, NeuroVue^® Red and NeuroVue^® Green and their use for double and triple labeling of neuronal profile. B Fritzsch et al. Brain Res Bull., 66, 3, 249-258 (2005).

A disorganized innervation of the inner ear persists in the absence of ErbB2. JK Morris et al. Brain Res.,1091:186-199 (2006).

Deafferentiation induces novel projections in the auditory brainstem after hearing onset. CY Hsieh and KS Cramer. Journal of Comparative Neurology, 497:589-599 (2006)

Al-Mehdi AB, Patel M, Haroon A, Reed D, Ohlsson-Wilhelm B, Muirhead K and Gray BD. Increased Depth of Cellular Imaging in the Intact Lung using Far Red and Near Infrared Fluorescent Probes. Int. J. Biomed Imaging. 1-7 (2006).

Stewart CC, Woodring ML, Podniesinski E and Gray BD. Flow cytometer in the infrared: inexpensive modifications to a commercial instrument. Cytometry, Part A, 67A, #2, 104-111, 2005

NeuroVue is a registered trademark of PTI Research, Inc. For patents see list.

转染技术新宠-Polysciences阳离子聚合物基因转染技术 其优点：适用宿主广泛，操作简便，细胞毒性小，转染率高。其中聚乙烯亚胺（PEI）的转染性能最好。聚乙烯亚胺是一种具有较高的阳离子电荷密度的有机大分子，每相隔两个碳原子，就是第三个原子都是质子化的氨基氮原子，使得聚合物网络在任何PH下都能充当有效的“质子海绵”体。这种聚阳离子能将各种报告基因转入各种种属细胞，其效果好于脂质聚酰胺，而且细胞毒性低。大量实验证明，PEI是非常有希望的基因治疗载体。目前在设计更复杂的基因载体时，PEI经常做为核心组成成分。