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Quantum™ Simply Cellular® Kits contain a mixture of microbeads which bind specific amounts of IgG antibodies. These standards are calibrated in terms of Antibody Binding Capacity (ABC) and can be used to construct calibration plots to determine the ABC of unknown samples and monitor instrument performance. Our FREE Flow Cytometry Data Analysis template, QuickCal®, is designed to work with all Quantum™ Simply Cellular® Kits (Access Number provided with your standards).
A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.
Available in three test size volumes:
A - 20 tests (1ml)
B - 100 tests (5ml)
C - 280 tests (2 x 7ml)
转染技术新宠-Polysciences阳离子聚合物基因转染技术 其优点:适用宿主广泛,操作简便,细胞毒性小,转染率高。其中聚乙烯亚胺(PEI)的转染性能最好。聚乙烯亚胺是一种具有较高的阳离子电荷密度的有机大分子,每相隔两个碳原子,就是第三个原子都是质子化的氨基氮原子,使得聚合物网络在任何PH下都能充当有效的“质子海绵”体。这种聚阳离子能将各种报告基因转入各种种属细胞,其效果好于脂质聚酰胺,而且细胞毒性低。大量实验证明,PEI是非常有希望的基因治疗载体。目前在设计更复杂的基因载体时,PEI经常做为核心组成成分。

