Fluoresbrite® carboxylate microspheres are fluorescent monodispersed polystyrene microspheres that have carboxylate groups on their surfaces which can be activated for the covalent coupling of proteins. Polysciences’ Fluoresbrite® particles are used worldwide in phagocytosis and neural retrograde transport studies, and as markers for cell bound antigens. These microspheres are packaged as 2.5% aqueous suspensions. YO has limited water solubility, and some leaching may occur with rigorous washing.

Nominal Reference Values:

• 3.00µm particles packaged as 2.5% aqueous suspension.
• 1.68 x 10⁹ particles/ml
• Diameter Coefficient of Variation (CV) = 5%
• Excitation max. = 529nm
• Emission max. = 546nm

转染技术新宠-Polysciences阳离子聚合物基因转染技术 其优点：适用宿主广泛，操作简便，细胞毒性小，转染率高。其中聚乙烯亚胺（PEI）的转染性能最好。聚乙烯亚胺是一种具有较高的阳离子电荷密度的有机大分子，每相隔两个碳原子，就是第三个原子都是质子化的氨基氮原子，使得聚合物网络在任何PH下都能充当有效的“质子海绵”体。这种聚阳离子能将各种报告基因转入各种种属细胞，其效果好于脂质聚酰胺，而且细胞毒性低。大量实验证明，PEI是非常有希望的基因治疗载体。目前在设计更复杂的基因载体时，PEI经常做为核心组成成分。