Overview
GainathoroughunderstandingofmiRfunction
PermanentlyknockdownmiRswithSBI’smiRZip™anti-miRNAExpressionLentivectors.LeveragingSBI’spowerfulandwell-regardedthird-generationlentivectortechnology,eachofourmiRZiplentivectorsexpressesashorthairpinRNA(shRNA)that,afterprocessing,preferentiallyproducesananti-sensemiRNA.Thehairpinisrationallydesignedtobeasymmetric,ensuringthatthesensestranddoesnotcontaintheendogenousmiRNAsequenceandenablingaccumulationoftheanti-miRNA.TheresultisrobustderepressionofthetranscriptstargetedbythemicroRNAbeing“zipped,”andelevationofthecorrespondingproteinlevels.
StudymiRswithSBI’spowerfulmiRZiptechnology:
- Stableandpermanentanti-microRNAexpressionfromaconstitutiveH1promoter
- Rationallydesigned,asymmetrichairpinsoptimizedforanti-sensemicroRNAproduction
- EfficientsuppressionofspecificendogenousmicroRNAs
- Reliabledeliverytodividingornon-dividingcells
- Selection/sortingfortransfected/transducedcellswitheithercopGFPorpuromycin
Conducthigh-throughputphenotypicscreenswithourpoolofpre-packagedlentiviralparticlescontainingourentiremiRZipcollection(Cat.#MZIPPLVA-1).
SupportingData
SeeSBI’smirZiptechnologyinaction
miRZipconstructsexpresshighlevelsofanti-microRNAs
Figure1.miRZipconstructsexpresshighlevelsofanti-microRNAs.miRZipconstructsweretransfectedintoHEK293cells,totalRNAisolated,andmiRZiplevelsmeasuredusingSBI’sQuantiMirAssay(Cat.#RA420A-1).ComparedtoQuantiMirassaysperformedonRNAisolatedfromnon-transfectedcells,themiRZip-transfectedcellsdeliveredhighexpressionlevelsofthespecificmiRZipanti-miRNAs.
EfficientmodulationoftargetproteinlevelsbySBI’sLenti-miRandmiRZiptechnologies
Figure2.TargetproteinlevelsareefficientlymodulatedbySBI’sLenti-miR-29aandmiRZip-29amicroRNAconstructs.
miRZiptechnologyiseffectiveinafunctionalassay
Figure3.miRZip-21andmiRZip-145exhibitanti-oncogenicandoncogenicactivity,respectively,inacellinvasionassayusingMDA-MB-231breastcancercells.(Toppanel)miR-21isanoncogenicmiRNAandmiR-145isatumorsuppressingmiRNA.TransductionofmiRZip-21(theanti-miR21lentiviralconstruct)andmiRZip-145(theanti-miR145lentiviralconstruct),inhibitedtherespectivemiRs,resultinginalowerpercentageofinvADIngcellsinmiRZip-21-transducedcellsandahigherpercentageofinvadingcellsinmiRZip-145-transducedcells.(Bottompanel)Additionally,themiRZip-145lentivirusinhibitedendogenousmiR-145andelevatedproteinexpressionlevelsofthemiR-145targetoncogenec-Myc.
AvailablemiRZip™anti-miRNAExpressionLentivectors
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