Our Anti-IBA1/AIF1 chicken primary antibody from AvesLabs is manufactured in-house. It detectsIBA1/AIF1, and is purified. It is provided in liquid form.

Volume: 100µL (IBA1-0020) or 500µL (IBA1-0100)

Concentration: 0.2mg/mL

Clonality: Polyclonal

Form: Purified

Host Species: Chicken

Antibody Isotype: IgY

Species Reactivity: Human, Mouse, Rat

Applications: ICC, WB

Immunogen: Purified recombinant human full-length IBA1/AIF1 (Uniprot P55008) expressed in E.Coli

Protein Name / Synonyms: Allograft inflammatory factor 1, AIF-1, Ionized calcium-binding adapter molecule 1, Protein G1

Target Description: AIF1, allograft inflammatory factor-1, (also known as Iba1, Ionized calcium binding adaptor molecule 1) is a 17 kDa protein that is a pro-inflammatory molecule that mediates calcium signals (Ito, D. et al 1998). During inflammation, increased expression of AIF1 occurs in microglia, macrophages, T-cells, synoviocytes, and adipocytes (Watano, K., et al, 2001). Expression of AIF-1 has been observed in healthy brains and varies throughout the day, gaining the highest expression in the prefrontal and parietal cortices when sleeping (Romcy-Pereira, R.N, et al, 2009). Increased expression of AIF1 has been observed in several diseases including breast cancer (Liu, S., et al 2008), atherosclerosis (Utans, U., et al, 1995), and rheumatoid arthritis (Kimura, M., et al, 2007).

Gene ID: AIF1 IBA1

Physical State: Liquid

Conjugate: Unconjugated

Production Notes: Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification

Validation and Application Notes

Molecular Weight: 17 kDa

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.

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二抗 该系列产品特点在于鸡IgY分子与哺乳动物IgG分子截然不同，因此由之制备的二抗不与来源于兔、小鼠或其他哺乳动物的一抗产生交叉反应，特别适用于组织学的免疫双标检测。该系列源于羊的二抗可交联荧光基团/HRP/生物素/琼脂糖，可广泛用于WB，免疫组化/免疫细胞化学及免疫共沉淀研究。