Details

Chickens were immunized with purified, non-denatured mouse IgG (containing both heavy and light chains). Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-mouse IgG’s were affinity-purified over a column conjugated with purified mouse IgG (containing both heavy and light chains) and then conjugated with horseradish peroxidase.

Volume: 1 mL

Concentration: 1 mg/mL

Physical State: Liquid

Buffer: Phosphate-buffered saline (PBS) (pH 7.2), sodium azide (0.02%,w/v) (as an anti-microbial agent).

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). For longer storage periods, dilute the antibodies with sterile glycerol and store at -20°C. Do not freeze these antibodies unless you want to store them for longer periods of time.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.

↑ back to top

二抗 该系列产品特点在于鸡IgY分子与哺乳动物IgG分子截然不同，因此由之制备的二抗不与来源于兔、小鼠或其他哺乳动物的一抗产生交叉反应，特别适用于组织学的免疫双标检测。该系列源于羊的二抗可交联荧光基团/HRP/生物素/琼脂糖，可广泛用于WB，免疫组化/免疫细胞化学及免疫共沉淀研究。