Our beta-galactosidase antibody recognizes the LacZ gene product with high titre and high specificity. Since this antibody was generated in chickens, it can be used together with your existing rabbit and mouse primary antibodies for double immunostaining with no secondary antibody cross reactivity. It even works well with fusion proteins of LacZ!

Volume: 100 µL (BGL-1010) or 400 µL (BGL-1040)

Concentration: 10 mg/mL

Clonality: Polyclonal

Form: Mixture of IgY fraction and affinity-purified antibodies

Host Species: Chicken

Applications: ICC, IHC, WB

Antibody Registry ID (RRID): AB_2313508

Physical State: Liquid

Production Notes: Chickens were immunized with purified ß-Galactosidase (lacZ) protein emulsified in Freund’s adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using ß-Galactosidase conjugated to an agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 µg/mL) and purified IgY (10 mg/mL), and is filter-sterilized.

Validation and Application Notes

Western Blot Dilution Range: 1:5000-1:10000

IHC Dilution Range: 1:2500-1:5000

Quality Control

This antibody preparation was analyzed by immunohistochemistry (at a dilution of 1:5000) using tissues infected with a virus bearing the LacZ gene. Secondary antibodies were fluorescein-labeled affinity-purified goat anti-chicken IgY (1:2000 dilution, Aves Labs Cat.# F-1005).

Storage

Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies at -80°C unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.

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二抗 该系列产品特点在于鸡IgY分子与哺乳动物IgG分子截然不同，因此由之制备的二抗不与来源于兔、小鼠或其他哺乳动物的一抗产生交叉反应，特别适用于组织学的免疫双标检测。该系列源于羊的二抗可交联荧光基团/HRP/生物素/琼脂糖，可广泛用于WB，免疫组化/免疫细胞化学及免疫共沉淀研究。