This product is an unlabeled, affinity-purified chicken anti-C-MYC epitope antibody.

Volume: 1 mL

Concentration: 100 µg/mL (based on absorbance at 280 nm)

Clonality: Polyclonal

Form: Affinity Purified

Host Species: Chicken

Applications: ELISA, ICC, IHC, IP, WB

Antibody Registry ID (RRID): AB_2313514

Physical State: Liquid

Buffer: Phosphate-buffered saline (PBS) (pH 7.2), sodium azide (0.02%, w/v) as an anti-microbial agent.

Production Notes: Chickens were immunized with a synthetic peptide containing the human c-myc epitope (i.e., EQKLISEEDL) conjugated to keyhole limpet hemocyanin. The first injection included complete Freund’s adjuvant; subsequent injections included incomplete Freund’s adjuvant. Eggs were collected after the fourth injection, and antibodies were purified from the yolks using a proprietary method that yields a purity of >90%. Antibodies were affinity-purified over a peptide column and then dialyzed against phosphate-buffered isotonic saline. Sodium azide was added and the antibodies were filter-sterilized (0.2 µm).

Validation and Application Notes

Western Blot Dilution Range: 1:500-1:1000

IHC Dilution Range: 1:100-1:250

Quality Control

Antibodies were analyzed using ELISA and Western Blots using an N-terminal C-MYC-tagged protein. Secondary antibodies used in these quality control assays were alkaline phosphatase- (1:1000 dilution) and HRP-labeled (1:3000 dilution) goat anti-chicken IgY antibodies (Aves Labs), respectively.

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). For longer storage periods, dilute the antibodies with sterile glycerol and store at -20°C. Do not freeze these antibodies unless you want to store them for longer periods of time.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.

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二抗 该系列产品特点在于鸡IgY分子与哺乳动物IgG分子截然不同，因此由之制备的二抗不与来源于兔、小鼠或其他哺乳动物的一抗产生交叉反应，特别适用于组织学的免疫双标检测。该系列源于羊的二抗可交联荧光基团/HRP/生物素/琼脂糖，可广泛用于WB，免疫组化/免疫细胞化学及免疫共沉淀研究。