Details

Chickens were immunized with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA) conjugated to keyhole limpet hemocyanin. After repeated injections, immune eggs were collected, from which the IgY fractions were prepared. These fractions were then affinity-purified using a peptide column, coupled to HRP, and filter-sterilized (0.45 µm).

Volume: 1 mL

Concentration: 100 µg/mL

Clonality: Polyclonal

Form: Affinity Purified

Host Species: Chicken

Applications: WB

Antibody Registry ID (RRID): AB_2313513

Physical State: Liquid

Validation and Application Notes

Western Blot Dilution Range: 1:5000

Quality Control

Antibodies were analyzed using ELISA and western blot analyses. By ELISA, the antibodies were diluted 1:10,000 and gave an optical density of >1.0 in a 15 minute reaction using tetramethyl benzidine as the substrate against peptide conjugated to a different carrier protein. By western blot, the antibodies were diluted 1:1,000 and gave a prominent band.

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). For longer storage periods, dilute the antibodies with sterile glycerol and store at -20°C. Do not freeze these antibodies unless you want to store them for longer periods of time.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.

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二抗 该系列产品特点在于鸡IgY分子与哺乳动物IgG分子截然不同，因此由之制备的二抗不与来源于兔、小鼠或其他哺乳动物的一抗产生交叉反应，特别适用于组织学的免疫双标检测。该系列源于羊的二抗可交联荧光基团/HRP/生物素/琼脂糖，可广泛用于WB，免疫组化/免疫细胞化学及免疫共沉淀研究。