Details

GoatswereimmunizedwithpurifiedchickenIgY(wholeprotein)emulsifiedinFreund’sadjuvant.Aftermultipleinjections,goatswerebledandserumcollected.Antibodieswereisolatedusingaffinitychromatographyandconjugatedtoalkalinephosphatase.

Volume:1mL

Concentration:0.1mg/mL

Clonality:Polyclonal

Form:AffinityPurified

HostSpecies:Goat

SpeciesReactivity:Chicken

Immunogen:IgY

PhysicalState:Liquid

Conjugate:AP

Buffer:HEPES(50mM,pH7.0),NaCl(0.1M),bovineserumalbumin(0.2%,w/v),MgCl2(1.0mM),ZnCl2(0.1mM),sodiumazide(0.02%,w/v)(asanantimicrobialagent).

Specificity:Doesnotcross-reactwithmammalianIgGs

QualityControl

Antibodieswereanalyzedusingimmunoelectrophoresis(seebelow)andasandwichELISA.Inthislattertest,a1:800dilutionofGGHL-30PH-2wasfoundtogenerateanO.Dof1.0ina20minutereactionwithp-nitrophenylphosphateasthesubstrate.

Storage

Storeat4°Cinthedark.Undertheseconditions,theantibodiesshouldhaveashelflifeofatleast12months(providedtheyremainsterile).Forlongerstorageperiods,dilutetheantibodieswithsterileglycerolandstoreat-20°C.Donotfreezetheseantibodiesunlessyouwanttostorethemforlongerperiodsoftime.

NOTE

AvesLabsproductsareintendedforuseasresearchlaboratoryreagents.Theyarenotintendedforuseasdiagnosticortherapeuticreagentsinhumans.

↑backtotop

二抗 该系列产品特点在于鸡IgY分子与哺乳动物IgG分子截然不同，因此由之制备的二抗不与来源于兔、小鼠或其他哺乳动物的一抗产生交叉反应，特别适用于组织学的免疫双标检测。该系列源于羊的二抗可交联荧光基团/HRP/生物素/琼脂糖，可广泛用于WB，免疫组化/免疫细胞化学及免疫共沉淀研究。