Volume: 1 mL

Physical State: Liquid

Buffer: Phosphate-buffered saline (PBS)(pH 7.2), sodium azide (0.02%, w/v) (as an anti-microbial agent).

Production Notes: Eggs from at least 20 chickens were purified from the yolks using a proprietary method that yields a purity of >90%. The IgY fraction was then dialyzed against phosphate-buffered isotonic saline. Sodium azide was then added and the preparation was filter-sterilized (0.45 μm filter).

Quality Control

Antibodies were analyzed by SDS-polyacrylamide gel electrophoresis. Heavy chains (80 kDa), truncated heavy chains (40 kDa) and light chains (25 kDa) were found to make up >95% of the Coomassie-stainable material.

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). For longer storage periods, dilute the antibodies with sterile glycerol and store at -20°C. Do not freeze these antibodies unless you want to store them for longer periods of time.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.

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二抗 该系列产品特点在于鸡IgY分子与哺乳动物IgG分子截然不同，因此由之制备的二抗不与来源于兔、小鼠或其他哺乳动物的一抗产生交叉反应，特别适用于组织学的免疫双标检测。该系列源于羊的二抗可交联荧光基团/HRP/生物素/琼脂糖，可广泛用于WB，免疫组化/免疫细胞化学及免疫共沉淀研究。