Details
Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to unique sequences within the metabotropic glutamate receptor type 2 and type 3 gene products, but was shared between the human (Q14416.2, Q14832.2 NCBI) and mouse (Q14B12.2, Q9QYS2.1, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluates adjusted to 200 µg/mL, and the preparation was filter-sterilized.
Volume: 1000 µL
Concentration: 200 µg/mL
Clonality: Polyclonal
Host Species: Chicken
Species Reactivity:Human, Mouse, Rat
Applications: ICC, IHC
Protein Name / Synonyms: Metabotropic glutamate receptor 2 (mGluR2), Metabotropic glutamate receptor 3 (mGluR3)
Gene ID: GRM2 GPRC1B MGLUR2 (mGluR2) and GRM3 GPRC1C MGLUR3 (mGluR3)
Antibody Registry ID (RRID): AB_2313542
Physical State: Liquid
Validation and Application Notes
IHC Dilution Range: 1:1000-1:2000
Quality Control
This anti-peptide antibody preparation was analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Storage
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.
NOTE
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.
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二抗 该系列产品特点在于鸡IgY分子与哺乳动物IgG分子截然不同，因此由之制备的二抗不与来源于兔、小鼠或其他哺乳动物的一抗产生交叉反应，特别适用于组织学的免疫双标检测。该系列源于羊的二抗可交联荧光基团/HRP/生物素/琼脂糖，可广泛用于WB，免疫组化/免疫细胞化学及免疫共沉淀研究。
