Transfer of Eukaryote Suspension Cultures-蚂蚁淘在线

Obtainacultureofmousefibroblastcellsinsuspensionculture.Thiswillbeasimpleculturewithminimalrequirements,andoneselectedforexcellentgrowthcharacteristics.Thetransferprocedurewillbesimilartothatforprokaryotes,withafewmajorchanges.First,alltransferswillbedoneinatissueculturehoodinordertomaximizeasepsis.Secondly,thecellswillbetransferredwithsiliconizedpipettesratherthanwireloops.Thesiliconepreventsadheranceofthecellstotheglasswallofthepipette.

Atissueculturehoodisadevicethathasairmovinginlayersandunderpositivepressure.Sincetheairisfiltered,itcontainsminimalnumbersofbacteriaorfungalspores,andsinceitisunderapositivepressure,thoseparticlesthatarepresentareblownoutofthehood.Thelayeringpreventsairborneorganismsfromsettlingontheworksurfaces.

Arrangethematerialsinfrontofyou,easilyaccessIBLethroughtheopeningofthetissueculturehood.Ensurethatanyalcoholsandwrappingpaperarekeptclearofthebunsenburner.Pre-sterilizethehoodbeforeuse,andusedisposablesterilegloves.

Loosenthecapofatissuecultureflaskandthecapofastockbottleoftissueculturemedia.6

Insertthetipofasterilepipetteintothestockbottleandremove10mlofmedia.Transferthemediatothetissuecultureflask.

Openthetopofthesuspensioncultureanduseasterile1.0mltransferpipettetoremovea1.0mlsampleoftheculture.Transferittothefreshmediainthecultureflask.Secureallcapsthathavebeenloosened.

Placethenewculturesinanincubatorat37°C.

>>> 更多资讯详情请访问蚂蚁淘商城

USP商品列表

图片/货号	产品名/品牌	价格/货期	操作
1458019	美国药典(美国药典委员会)/新霉素B（150毫克）/1458019 USP	￥3000.00 3-4周	我要询价立即购买
1705378	USP//1705378 USP	￥9960.00 3-4周	我要询价立即购买
1500207	USP/帕罗西汀相关化合物E（50 mg）（4-（4-氟苯基）-1-甲基-1,2,3,6-四氢吡啶盐酸盐）/1500207 USP	￥11040.00 3-4周	我要询价立即购买
1081750	USP/1,4-丁二磺酸（120 mg）/1081750 USP	￥6000.00 3-4周	我要询价立即购买