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蚂蚁淘在线 / 品牌中心 / UBPBio / ubpbio/Rapid 26S蛋白酶体纯化试剂盒-L/J4320/1试剂盒

ubpbio/Rapid 26S蛋白酶体纯化试剂盒-L/J4320/1试剂盒

价格
￥13500.00
货号：J4320
浏览量：127
品牌：UBPBio
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商品描述
TheRapid26SProteasomePurificationKitisdesignedtorapidlypurifytheendogenous26Sproteasomefromcellortissueextractswithin8hoursaccordingtoapublishedmethod(reference1)withmodifications.ThisapproachusestheN-terminalubiquitin-like(Ubl)domainofhumanRAD23Basanaffinitybait,whichallowstherapidandgentleisolationofendogenous26Sproteasomes.Thebound26SproteasomeissubsequentlyelutedusingtheC-terminaltwoubiquitin-interactingmotifs(UIM)ofhumanS5a.

AdditionalInformation

Details

Thiskitissufficientforpurificationofthe26Sproteasomefrom300mg,6X50mgor30X10mgcellortissueextractsataconcentrationof5mg/ml.Usually,1-2%oftotalcellularproteinsarethe26Sproteasome.
Description
TheRapid26SProteasomePurificationKitisdesignedtorapidlypurifytheendogenous26Sproteasomefromcellortissueextractswithin8hoursaccordingtoapublishedmethod(reference1)withmodifications.ThisapproachusestheN-terminalubiquitin-like(Ubl)domainofhumanRAD23Basanaffinitybait,whichallowstherapidandgentleisolationofendogenous26Sproteasomes.Thebound26SproteasomeissubsequentlyelutedusingtheC-terminaltwoubiquitin-interactingmotifs(UIM)ofhumanS5a.
Components
•GST-Ubl(4mg/ml)...................................................2X1.2ml•GST(4mg/ml).........................................................2X1.2ml
•6xHis-UIM(4mg/ml)....................................................1X1ml•20XLysisBuffer........................................................3X1.2ml
•NaCl(1M)...............................................................2X1.25ml•ATP(500mM).................................................................0.5ml
•SUC-LLVY-AMC(50mM)..................................................30µl•GlutathioneAgaroseResin(75%slurry)...........2X1.0ml
•NickelXPureAgaroseResin(50%slurry)..........2X500µl
Images(Clickimagetoenlarge)

A.CoomassiestainedSDS-PAGEof5μgpurifiedGST-Ubl.B.CoomassiestainedSDS-PAGEof5μgpurified6xHis-UIM.C.CoomassiestainedSDS-PAGEof3μgpurifiedhuman26Sproteasomes.
D.Coomassiestainednative-PAGEof3μgpurifiedhuman26Sproteasomes.E.Solutionpeptidaseassayofthepurifiedhuman26Sproteasome.150μlreactionmixturescontained10nMpurifiedhuman26Sproteasomein50μMSUC-LLVY-AMCin20mMTris,pH7.1at37°C,2mMμME.ThereleasedAMCfluorescencewasmeasuredusingaplatereaderwithexcitationandemissionfiltersat360/40and460/30nm,respectively.Thisassaywasusedtomonitorthechymotrypsin-likeactivityofthe26Sproteasome.(Clickimagetoenlarge)
ProductName:Rapid26SProteasomePurificationKit-L
AlsoKnownAs:N/A
CatalogNo.:J4320
Size:1Kit
MolecularWeight:N/A
Species:N/A
Source:N/A
Stock:Enzymestockedin20mMTris,150mMNaCl,2mMβME,10%Glycerol;SubstratestockedinDMSO
Concentration:Seetubelabel
QualityAssurance:Enzyme>95%bySDS-PAGE;Substrate>98%byHPLCandNMR
Storage:Storeat-80°C;avoidmultiplefreeze-thawcycles
PDFDataSheet:PDFdatasheet,MSDS
NCBIRefSeq:N/A
Image(s):(Clickimagetoenlarge)
Schematicof26Sproteasomeenrichment
ShippingMethod:Dryiceshipping
References:1.BescheHC,HaasW,GygiSP,GoldbergAL.Biochemistry.2009;48:2538-49.
2.ElsasserS,SchmidtM,FinleyD.MethodsEnzymol.2005;398:353-63.
3.KisselevAF,GoldbergAL.MethodsEnzymol.2005;398:364-78.
Schematicof26Sproteasomeenrichment
Ubiquitin-Proteasome Biotechnologies成立于2011年，ubpbio是由科学家和投资者创立的私营企业。ubpbio致力于为学术界和工业界提供高质量的试剂和其他相关产品。ubpbio产品线包括的高度纯化的蛋白酶，E1和E2的酶，泛素和泛素衍生物，蛋白酶体抑制剂和蛋白酶基板。ubpbio不断开发创新以增加新的产品，ubpbio致力为全球的客户提供更多的帮助。在人类细胞中，有超过1000的蛋白质/酶，参与维持泛素-蛋白酶体系统的正常活动。失调的UPS已发现涉及大量的人类疾病包括癌症的病理生理，炎性疾病和神经退行性疾病。ubpbio致力为客户提供更好的泛素-蛋白酶体系统解决方案。