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公司地址
苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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https://www.ebiomall.com
商品描述
(Suc-LLVY)2-Rhodamineisafluorogenicsubstrateforthechymotrypsin–likeactivityofthe20Sand26Sproteasomes.Workingconcentrationsofthissubstrateis20-100µM.ThereleasedRhodamine110fluorescencecanbedetectedbyafluorimeterorplatereaderatexcitation/emissionof495nm/520nm,respectively.ComparedtoAMC,Rhodamine110isexcitedatlongerwavelengththatcansignificantlyenhancethesignal/noiseratio.ThissubstrateisextremlyvaluableforscreeningsmallchemicalinhibitorsofproteasomesbyavoidingtheinterferencefromchemicalcompoundautofluorescencethatoftenoccursatshorterwavelengthsaroundUVornear-UVregions.
Whenusedtodetermineproteasomeactivityincelllysates,celllysatesthatarepre-treatedwithaproteasomeinhibitorsuchasMG132,PS341orepoxomicincanbeusedtodeterminethefluorescencecontributedbyothercellularproteasesthatcleavethissubstrate.ReADIngsfromproteasomeinhibitor-treatedlysatesshouldbesubtracted.
Whenusedtodetermineproteasomeactivityincelllysates,celllysatesthatarepre-treatedwithaproteasomeinhibitorsuchasMG132,PS341orepoxomicincanbeusedtodeterminethefluorescencecontributedbyothercellularproteasesthatcleavethissubstrate.ReADIngsfromproteasomeinhibitor-treatedlysatesshouldbesubtracted.
AdditionalInformation
| ProductName: | (Suc-Leu-Leu-Val-Tyr)2-Rhodamine110 |
|---|---|
| AlsoKnownAs: | (Suc-LLVY)2-Rhodamine110 |
| CatalogNo.: | G1110 |
| Size: | 2mg |
| MolecularWeight: | 1507.7Da |
| Species: | N/A |
| Source: | Synthetic |
| Stock: | Powder |
| Concentration: | N/A |
| QualityAssurance: | >98%byHPLCandNMR |
| Storage: | EligIBLeforroomtemperatureshipping.Storeat-80°Cuponreceiving;avoidmultiplefreeze-thawcyclesafterdissolvinginDMSO. |
| PDFDataSheet: | DownloadPDFdatasheet,MSDS |
| NCBIRefSeq: | N/A |
| Image(s): | No |
| ShippingMethod: | Roomtemperatureshipping |
| References: | No |
