ubpbio/K29/K33多泛素链捕获试剂盒/J4470/1试剂盒

价格
¥7600.00
货号:J4470
浏览量:127
品牌:UBPBio
服务
全国联保
正品保证
正规发票
签订合同
商品描述
Thiskitisdesignedforenrichmentofcellularproteinsconjugatedwithlysine29/33-linkedpolyubiquitinchainsusingGST-TRABID(ZNF1).PolyubiquitinatedproteinsboundonGST-TRABID(ZNF1)canbeprecipi-tatedusingglutathioneresinandelutedbyabuffercontaining10mMglutathione.Enrichedpolyubiqui-tinatedproteinscanbeassayedbyimmunoblottingormassspectrometry.

AdditionalInformation

Details

Description
Thiskitisdesignedforenrichmentofcellularproteinsconjugatedwithlysine29/33-linkedpolyubiquitinchainsusingGST-TRABID(ZNF1).PolyubiquitinatedproteinsboundonGST-TRABID(ZNF1)canbeprecipi-tatedusingglutathioneresinandelutedbyabuffercontaining10mMglutathione.Enrichedpolyubiqui-tinatedproteinscanbeassayedbyimmunoblottingormassspectrometry.
Components
•GST-TRABID(ZNF1)(2mg/mleach)......................................1ml
•GST(4mg/ml)..........................................................................0.5ml•Iodoacetamine(IAA).................................................................25mg
•Glutathione(reduced)..............................................................100mg•GlutathioneAgaroseResin(75%slurry)..................................1.0ml
Images:
(Clickimagetoenlarge)
ProductName:K29/K33PolyubiquitinChainCaptureKit
AlsoKnownAs:N/A
CatalogNo.:J4470
Size:1Kit
MolecularWeight:N/A
Species:N/A
Source:N/A
Stock:Enzymestockedin20mMTris,150mMNaCl,2mMβME,10%Glycerol
Concentration:Seetubelabel
QualityAssurance:Enzyme>95%bySDS-PAGE
Storage:Storeat-80°C;avoidmultiplefreeze-thawcycles
PDFDataSheet:PDFdatasheet
NCBIRefSeq:N/A
Image(s):
Images:(Clickimagetoenlarge)
AprocedureforcapturingK29/K33polyubiquitinatedproteinsincellortissuelysates

GST-TRABID(NZF1)pulldownassay
1.20ugcelllysates2.GSTpulldown
3.GST-TRABID(NZF1)pulldown

ShippingMethod:Dryiceshipping
References:N/A
AprocedureforcapturingK29/K33polyubiquitinatedproteinsincellortissuelysates

GST-TRABID(NZF1)pulldownassay
1.20ugcelllysates2.GSTpulldown
3.GST-TRABID(NZF1)pulldown

Ubiquitin-Proteasome Biotechnologies成立于2011年,ubpbio是由科学家和投资者创立的私营企业。ubpbio致力于为学术界和工业界提供高质量的试剂和其他相关产品。ubpbio产品线包括的高度纯化的蛋白酶,E1和E2的酶,泛素和泛素衍生物,蛋白酶体抑制剂和蛋白酶基板。ubpbio不断开发创新以增加新的产品,ubpbio致力为全球的客户提供更多的帮助。在人类细胞中,有超过1000的蛋白质/酶,参与维持泛素-蛋白酶体系统的正常活动。失调的UPS已发现涉及大量的人类疾病包括癌症的病理生理,炎性疾病和神经退行性疾病。ubpbio致力为客户提供更好的泛素-蛋白酶体系统解决方案。