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商品描述
Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 µM. In Ub(K48 only), all lysine residues but lysine 48 were substituted with arginines, thereby it only supports the formation of lysine 48-linked polyubiquitin chains.
Additional Information
| Product Name: | 6xHis-Ubiquitin (K48 only) |
|---|---|
| Also Known As: | 6xHis-[K48-only]Ub |
| Catalog No.: | E1870 |
| Size: | 1 mg |
| Molecular Weight: | 8.5 kDa |
| Species: | Human |
| Source: | Bacterial recombinant |
| Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol |
| Concentration: | See tube label |
| Quality Assurance: | ~95% by SDS-PAGE, see datasheet for gel image |
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles |
| PDF Data Sheet: | PDF Datasheet， MSDS |
| NCBI RefSeq: | N/A |
| Image(s): | No |
| Shipping Method: | Dry ice shipping |
| References: | 1. Hershko A, et al. (1980) Proc Natl Acad Sci USA 77(4), 1783 – 1786. 2. Pickart CM (1997) FASEB 11(13), 1055 – 1066. 3. Hershko A, et al. (1998) Ann Rev Biochem 67, 425 – 479. 4. Jiang X, et al. (2012) Nature Reviews Immunology 12, 35 – 48. 5. Bremm A, et al. (2012) Methods Mol Biol 832, 291 – 228. |
Details
Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 µM. In Ub(K48 only), all lysine residues but lysine 48 were substituted with arginines, thereby it only supports the formation of lysine 48-linked polyubiquitin chains.
Images (Click image to enlarge)
Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified 6xHis-Ubiquitin (K0)
Lane 3: 5 µg purified 6xHis-Ubiquitin (K6 only) Lane 4: 5 µg purified 6xHis-Ubiquitin (K11 only)
Lane 5: 5 µg purified 6xHis-Ubiquitin (K27 only) Lane 6: 5 µg purified 6xHis-Ubiquitin (K29 only)
Lane 7: 5 µg purified 6xHis-Ubiquitin (K33 only) Lane 8: 5 µg purified 6xHis-Ubiquitin (K48 only)
Lane 9: 5 µg purified 6xHis-Ubiquitin (K63 only)
Ubiquitin-Proteasome Biotechnologies成立于2011年，ubpbio是由科学家和投资者创立的私营企业。ubpbio致力于为学术界和工业界提供高质量的试剂和其他相关产品。ubpbio产品线包括的高度纯化的蛋白酶，E1和E2的酶，泛素和泛素衍生物，蛋白酶体抑制剂和蛋白酶基板。ubpbio不断开发创新以增加新的产品，ubpbio致力为全球的客户提供更多的帮助。在人类细胞中，有超过1000的蛋白质/酶，参与维持泛素-蛋白酶体系统的正常活动。失调的UPS已发现涉及大量的人类疾病包括癌症的病理生理，炎性疾病和神经退行性疾病。ubpbio致力为客户提供更好的泛素-蛋白酶体系统解决方案。 Images (Click image to enlarge)
Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified 6xHis-Ubiquitin (K0)
Lane 3: 5 µg purified 6xHis-Ubiquitin (K6 only) Lane 4: 5 µg purified 6xHis-Ubiquitin (K11 only)
Lane 5: 5 µg purified 6xHis-Ubiquitin (K27 only) Lane 6: 5 µg purified 6xHis-Ubiquitin (K29 only)
Lane 7: 5 µg purified 6xHis-Ubiquitin (K33 only) Lane 8: 5 µg purified 6xHis-Ubiquitin (K48 only)
Lane 9: 5 µg purified 6xHis-Ubiquitin (K63 only)
