Description

TheCultreCoat®VascularPermeABIlityAssayemploysasimplifiedBoydenchamberdesignwithaonemicronpolyethyleneterephthalate(PET)membranecoatedwithcollagenI.Portswithinthemigrationchamber(top)allowaccesstotheassaychamber(bottom)withoutdismantlingthedevice.Thisdesigniseasiertouse,preventscontamination,andisadaptableforrobotichighthroughputsystems.Theassaychambermaybedirectlyanalyzedinaplatereader,eliminatingtransferstepsthatintroduceadditionalvariabilitytotheassay.

Theconfluentcellmonolayerformsabarrierthroughcell-celladhesionmechanismssuchasjunctions(adherens,tight,andgap)anddesmosomes,andthisbarrierrestrictsFITC-Dextrantotheinserts.Disruptionofthesecell-celladhesionmechanismscreatesgapsintheintercellularspacethatallowdiffusionofFITC-Dextranintothebottomchamber,sotheamountofFITC-Dextranthatdiffusesisrelatedtotheamountofintercellularspace,whichisrepresentativeofvascularpermeability.

Catalog#3475-096-Kincludes:
CatalogNumber	Description	Qty
3475-096-01	CultreCoat®96WellVascularPermeabilityChamber	1
3475-096-02	CultreCoat®FITC-Dextran	1

Catalog#3475-024-Kincludes:
CatalogNumber	Description	Qty
3475-024-01	CultreCoat®24WellVascularPermeabilityChamber	1
3475-096-02	CultreCoat®FITC-Dextran	1

Trevigen，Inc.是一家发展迅速的生物技术公司，致力于开发用于癌症研究，药物发现，遗传毒理学，再生医学和干细胞研究的产品和技术。我们提供试剂盒和试剂，用于研究DNA损伤和修复，细胞凋亡，氧化应激，癌细胞行为，3D培养和干细胞。该公司获得了美国国立卫生研究院（National Institutes of Health）多项SBIR资助。