Description

NOTE: StartingJanuary1st,2017freesamplesizesof Cultrex® OrganoidHarvestingSolution, Cultrex®BMEType2, and Cultrex®BMETypeR1 willbeaddedtoyourorder. Theseproductsarealsosoldindividually,moreinformationonTrevigen®’sCultrexOrganoidproductscanbefoundhere.

Roofplate-specificSpondin-1(R-Spondin1orRspo1),alsoknownasCRISTIN3,isa27kDasecretedactivatorproteinthatbelongstotheR-Spondinfamily.R-SpondinspositivelyregulateWnt/beta-cateninsignaling,mostlikelybyactingasaligandforLGR4-6receptorsandaninhibitorforZNRF3.R-Spondin-1inducesproliferationofintestinalcryptepithelialcells,increasesintestinalepithelialhealing,andsupportsintestinalepithelialstemcellrenewal.

Features:

• RobustandstableexpressionofR-Spondin1.
• Twoepitopetagsprovideflexibilitytopurifyanddetecttheexpressedprotein.
• AccesIBLesourceofeitherpurifiedR-Spondin1orR-Spondin1conditionedmedium.
• Suitabletosupplementbothhumanandmurineorganoidculturemedium.

Qualitycontrolspecifications:

• NegativebyPCRforMycoplasma.
• ExpressiondetectedbyWesternBlot–Abandofapproximately70kDacorrespondingtoHA-R-Spondin1-Fcisdetectedintheconditionedmediumofcellsafter7daysofculture.
• BIOLOGicalactivitydeterminedforeachlot:
  • TopFlashassay–HA-R-Spondin1-FcmustinducetheexpressionofluciferaseunderthecontrolofaTCF/LEFpromoter.

Trevigen，Inc.是一家发展迅速的生物技术公司，致力于开发用于癌症研究，药物发现，遗传毒理学，再生医学和干细胞研究的产品和技术。我们提供试剂盒和试剂，用于研究DNA损伤和修复，细胞凋亡，氧化应激，癌细胞行为，3D培养和干细胞。该公司获得了美国国立卫生研究院（National Institutes of Health）多项SBIR资助。