Trevigen/gamma-H2AX药效学分析试剂盒/4418-096-K/96试验

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¥25000.00
货号:4418-096-K
浏览量:127
品牌:Trevigen
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Description

4418-096-k_calculation_worksheet

InanELISAassay,immobilizedgamma-H2AXantibodyinthewellsofa96-wellplatecapturesgamma-H2AXfromsamplelysate. IncubationwithaH2AXdetectingantibody,followedbyadditionofagoatanti-mouseHRPconjugateandachemiluminescentHRPsubstrateyieldsrelativelightunits(RLU)thatdirectlycorrelateswiththeamountofgamma-H2AXinthesample.Thegamma-H2AXpolyclonalantibodymaybepurchasedseparately.

FEATURES:

  • Chemiluminescent,non-rADIoactiveELISA
  • Pre-coated96wellcaptureantibodyplate
  • Dynamicrangefrom10pMto800pM
  • Sensitivitywith5pMofgamma-H2AX

APPLICATIONS:
Quantitationofgamma-H2AXlevelsinperipheralbloodmononuclearcells,tissueculturecells,andtissuebiopsies.

Catalog#4418-096-Kincludes:
CatalogNumberDescriptionQty
4418-096-01Gamma-H2AXStandard,1µM1
4418-096-06JurkatCellLysateControl1
4418-096-02AssayBuffer1
4418-096-05CellLysisReagent1
4418-096-0725XWashBuffer1
4418-096-P96-Stripwellpre-coatedplatewith3filmsealers1
4675-096-01PARPPeroxyGlow™A1
4675-096-02PARPPeroxyGlow™B1
4418-096-03H2AXIgMDetectingAntibody1
4418-096-04Goatanti-MouseIgMHRPConjugate1

Abstract2423:DevelopmentofavalidatedhighthroughputELISAassayforgammaH2AXasapharmacodynamicMarker
JayGeorgeandHaiXu
CancerRes.,Oct2014;74:2423.
http://cancerres.aacrjournals.org/cgi/content/abstract/74/19_Supplement/2423}

Engineeredcellulargene-replacementplatformforselectiveandinducIBLeproteolyticprofiling
CharlesW.Morgan,JuanE.Diaz,SamanthaG.Zeitlin,DanielC.Gray,andJamesA.Wells
PNAS,Jul2015;112:8344–8349.
http://www.pnas.org/cgi/content/abstract/112/27/8344

Trevigen,Inc.是一家发展迅速的生物技术公司,致力于开发用于癌症研究,药物发现,遗传毒理学,再生医学和干细胞研究的产品和技术。我们提供试剂盒和试剂,用于研究DNA损伤和修复,细胞凋亡,氧化应激,癌细胞行为,3D培养和干细胞。该公司获得了美国国立卫生研究院(National Institutes of Health)多项SBIR资助。