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蚂蚁淘在线 / 品牌中心 / Trevigen / Trevigen/超氧化物歧化酶检测试剂盒（试管）/7500-100-K/100反应

Trevigen/超氧化物歧化酶检测试剂盒（试管）/7500-100-K/100反应

价格
￥10560.00
货号：7500-100-K
浏览量：127
品牌：Trevigen
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商品描述

Description

IntheSuperoxideDismutaseAssay,ionsgeneratedfromtheconversionofxanthinetouricacid,andhydrogenperoxidebyxanthineoxidase(XOD),convertNBTtoNBT-diformazan.NBT-diformazanabsorbslightat550nm.SODsreducesuperoxideionconcentrationsandtherebylowertherateofNBT-diformazanformation.TheextentofreductionintheappearanceofNBT-diformazanisameasureofSODactivitypresentinexperimentalsamples.Theassayisfreeofinterferencebyothercatalyticactivities,andisidealforassayingSODinmammaliancelllysates.Thekitcontainstheproperlysisbufferandthereagentsneededfor100experimentaltests,50positivecontrols,and50negativecontrols.UnlikesomeotherassaykitsforSOD,thissystemisnotgreatlydisturbedbytracemetals.Eachassayrequiresonlyaboutfiveminutes,andafterasimplecalculation,thepercentinhibitionoftheformationofNBT-diformazanbySODisconvertedtotherelativeactivityofthesample.

FEATURES:

  • Suitableformammaliancells.
  • Eachsampletakesonly5minutes.
  • ContainsSODfor50positivecontrols.
  • Suitablefortheassayof(Mn2+)-SOD,(Fe2+)-SOD,and(Cu/Zn)–SOD.

APPLICATIONS:

CalculatingSODactivitybymeasuringthereductionofNBT-diformazanincellextracts.

 

Catalog#7500-100-Kincludes:
CatalogNumberDescriptionQty
7500-100-01SuperOxideDismutase1
7500-100-05XODSolution1
7500-100-0225XSODReactionBuffer1
7500-100-03XanthineSolution1
7500-100-04NBTSolution1
7500-100-0620XCellLysisSolution1

RosiglitazoneCausesCardiotoxicityviaPeroxisomeProliferator-ActivatedReceptor{gamma}-IndependentMitochondrialOxidativeStressinMouseHearts
HuameiHe,HaiTao,HuiXiong,ShengZhongDuan,FrancisX.McGowan,Jr.,RichardM.Mortensen,andJamesA.Balschi
Toxicol.Sci.,Apr2014;138:468–481.
http://toxsci.oxfordjournals.org/cgi/content/abstract/138/2/468


Protectiveactivityofanovelresveratrolanalogue,HS-1793,againstDNAdamagein137Cs-irrADIatedCHO-K1cells
Jeong,M.H.,Yang,K.M.,Jeong,D.H.,Lee,C.G.,Oh,S.J.,Jeong,S.K.,Lee,K.W.,Jo,Y.R.andJo,W.S.
J.RadiatRes,May2014;55:464–47
http://jrr.oxfordjournals.org/cgi/content/abstract/55/3/464

Trevigen，Inc.是一家发展迅速的生物技术公司，致力于开发用于癌症研究，药物发现，遗传毒理学，再生医学和干细胞研究的产品和技术。我们提供试剂盒和试剂，用于研究DNA损伤和修复，细胞凋亡，氧化应激，癌细胞行为，3D培养和干细胞。该公司获得了美国国立卫生研究院（National Institutes of Health）多项SBIR资助。