Description

Thekithasbeendevelopedtoovercomethecommondifficultiesuniquetoneuronalsamplesincludingthefragilenatureofbraintissuesections,highbackgroundproblems,poorcounterstainingwithcommondyes,andtheneedtoperformduallabelingexperimentstodetectcellspecificantigensinconjunctionwithapoptoticcells.AkeyfeatureisNeuroPore™,aproprietarypermeABIlizationreagentthatgentlypermeabilizessampleswhileretainingcellmorphology.NeuroPore™alsocontainsblockingreagentstoallowitsuseasanantibodydiluentinimmunohistochemistryandtoreducebackgroundstaining.

DNAfragmentsgeneratedbyapoptosisareend-labeledwithmodifiednucleotidesusingahighlypurifiedterminaldeoxynucleotidyltransferaseenzyme(TdT).TheincorporatednucleotidesaredetectedusingahorserADIshperoxidasesystemthatcatalyzestheconversionofdiaminobenzidine(DAB)intoavisIBLedarkbrownprecipitate.NeuroTACS™IIcontainstheBlueCounterstaintoallowvisualizationofallcellswithinthesamplewithgoodcontrasttothebrownDABprecipitate.ADABenhancerisalsoprovidedwiththekitfortheoptiontointensifyordarkenDABstaining.Inaddition,theBlueCounterstainiscompatiblewiththered-coloredsubstratesusedforphosphatasedetectionindoublelabelingexperiments.Theprotocolincludesdetailsforlabelinginsituforapoptosisandantigendetectiononthesamesample.Toensurethatyourownsampleshavebeenprocessedandpermeabilizedcorrectly,weprovideTACS-Nuclease™,auniquereagentusedtogenerateapositivecontrolwithyourownsamples.Thisprovidesahighdegreeofconfidencefordatainterpretationandcanhelppinpointproblemstepsinthelabelingprocedure.

FEATURES:

• Uniquebuffersystemproducesmoreconsistentlabeling.
• Performancetestedonbrainsections.
• IncludesexclusiveNeuroPore™permeabilizationreagent.
• IncludesTACS-Nuclease™solutionforpreparingsample-dependentpositivecontrols.
• Helpsresolveuniqueproblemsencounteredwhendetectingapoptoticneuronalcells.

 

APPLICATIONS:

• Insitudetectionofapoptosisinfixedfrozen,paraffinembedded,orplasticembeddedcellsandtissues.
• Assistsintheidentificationofapoptoticmorphologies.

Catalog#4823-30-Kincludes:
CatalogNumber	Description	Qty
4800-30-01	ProteinaseK	1
4800-30-06	Streptavidin-HRP	1
4800-30-07	Diaminobenzidine	1
4800-30-09	DABEnhancer	1
4800-30-15	TACS-Nuclease™	1
4800-30-16	TACS-Nuclease™Buffer	1
4810-30-02	TACS®2TdTLabelingBuffer	1
4810-30-03	TACS®2TdTStopBuffer	1
4810-30-04	TACS®2TdTdNTP	1
4810-30-05	TdTEnzyme	1
4810-30-14	50xManganeseCation	1
4820-30-01	NeuroPore™	1
4820-30-13	BlueCounterstain	1

 

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Trevigen，Inc.是一家发展迅速的生物技术公司，致力于开发用于癌症研究，药物发现，遗传毒理学，再生医学和干细胞研究的产品和技术。我们提供试剂盒和试剂，用于研究DNA损伤和修复，细胞凋亡，氧化应激，癌细胞行为，3D培养和干细胞。该公司获得了美国国立卫生研究院（National Institutes of Health）多项SBIR资助。