Description

3DCulturesexhibitcellularbehaviorsandmorphologiessimilartothoseseeninvivo;however,theadaptationofthesemodelsforstudyingbiochemicalprocesseshasbeenimpededbythechallengeofseparatingintactcellsfromextracellularmatrixhydrogels. Commonly,proteasesareemployedtodegradetheseextracellularproteins;however,proteasesalsodegradeproteinsonthecellsurfaceandmaycarryoverintolysatepreparations.Non-enzymaticmethodologieshavealsobeendescribedfordepolymerizing extracellularmatrixproteins,althoughtheimplementationoftheseprotocolsremainsproblematicforsomeresearchers.TheCultrex®3DCulture10XCellWashBufferprepares BMEandLamininIhydrogels fortheisolationof3Dstructures.

Trevigen，Inc.是一家发展迅速的生物技术公司，致力于开发用于癌症研究，药物发现，遗传毒理学，再生医学和干细胞研究的产品和技术。我们提供试剂盒和试剂，用于研究DNA损伤和修复，细胞凋亡，氧化应激，癌细胞行为，3D培养和干细胞。该公司获得了美国国立卫生研究院（National Institutes of Health）多项SBIR资助。