Components:

The PromoCell 3D Tumorsphere Medium XF consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete medium.

Key features:

• Establish 3D tumorsphere cultures directly from 2D cancer cell lines
• Suitable for long-term routine culture of tumorspheres
• Compatible with most commonly used cancer cell lines
• Xeno-free and serum-free formulation

List of cell types tested for serial passage with the PromoCell 3D Tumorsphere Medium XF:

Tissue	Tested Cell Lines	Cell Lines Origin
Brain	U-87 MG	grade IV glioblastoma / astrocytoma of the human brain
Breast	MCF-7	pleural effusion of metastatic human breast adenocarcinoma
Breast	MDA-MB-231	pleural effusion of metastatic human breast adenocarcinoma (triple-negative)
Colon	HT-29	human colon adenocarcinoma
Connective tissue	HT1080	human fibrosarcoma
Liver	HepG2	hepatocellular carcinoma of the human liver
Lung	A-549	human lung carcinoma
Pancreas	Panc-1	epithelioid carcinoma of the human pancreatic duct
Prostate	LNCaP	lymph node metastasis of human prostate adenocarcinoma
Skin	A-431	epidermoid carcinoma of the human skin

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