The PromoCell Cancer Cell Line Medium XF is a cell culture medium developed for the standardized in vitro cultivation of established human cancer cell lines. Its serum-free and xeno-free formulation provides a culture environment devoid of all stimuli originating from non-defined materials. Thus, it has no ill-defined components such as fetal calf serum, extracts or hydrolysates and exhibits very low lot-to-lot variability.

Components:The PromoCell Cancer Cell Line Medium XF consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete medium

Note: The serum- and xeno-free formulation means non-availability of attachment factors. Hence, coating with an appropriate adhesion factor is a pre-requisite. For the establishment of the culture conditions, it is recommended to test fibronectin (e.g. Human Fibronectin Solution (C-43060)) and vitronectin (e.g. Vitronectin (C-69201)) coating.

Key features:

• Suitable for long-term routine culture of adherently growing established human cancer cell lines
• Compatible with most commonly used human cancer cell lines
• Xeno-free and serum-free formulation

List of cell types tested for serial passage with the PromoCell Cancer Cell Line Medium XF:

Tissue	Tested Cell Lines	Cell Lines Origin	Remarks
Brain	BV2	immortalized murine primary microglial cells	Coat with Fibronectin:1 µg/cm2
Breast	MCF-7	pleural effusion of metastatic human breast adenocarcinoma	Coat with Fibronectin:1 µg/cm2
Colon	HT-29	human colon adenocarcinoma	Coat with Vitronectin:0.5 µg/cm2
Connective tissue	HT1080	human fibrosarcoma	Coat with Fibronectin:1 µg/cm2
Liver	HepG2	hepatocellular carcinoma of the human liver	Coat with Vitronectin:0.5 µg/cm2
Lung	A-549	human lung carcinoma	Coat with Vitronectin:0.5 µg/cm2
Prostate	LNCaP	lymph node metastasis of human prostate adenocarcinoma	3D culture in C-28070 is recommended
Peripheral blood	MV-4-11	Human acute myelogenous leukemia (suspension)	No coating required
Bone marrow	KG-1	Human acute myelogenous leukemia (suspension)	No coating required
Kidney	ACHN	Human Renal Cell Carcinoma	Coat with Fibronectin:1 µg/cm2
Brain	A172	Human Glioblastoma	Coat with Fibronectin:1 µg/cm2
Skeletal muscle	C2C12	Mouse Myoblasts	Coat with Fibronectin:1 µg/cm2
Skin	B16-F10	Mouse Melanoma	Coat with Fibronectin:1 µg/cm2
Abelson murine leukemia virus-induced tumor	RAW264.7	Mouse Macrophage	Coat with Fibronectin:1 µg/cm2
Brain, neuroectodermal	Neuro-2a	Mouse Neuroblastoma	Coat with Fibronectin:1 µg/cm2

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