Antigen/Purification: Collapse
The antigen is a peptide corresponding to amino acid residues from the C-terminal region of rat stargazin, conjugated to keyhole limpet hemocyanin (KLH).
The antibody is prepared from pooled rabbit serum by affinity purification via chromatography on an affinity column to which the C-terminal peptide used as immunogen was coupled.
Biological Significance: Collapse
Stargazin is a member of the transmembrane AMPAR regulatory proteins (TARP) family and is involved in glutamate receptor trafficking. It has been recently demonstrated (Tomita et al., 2005; Priel et al., 2005) that the interaction between stargazin and AMPA receptors is critical for the correct localization of the receptors at the synapse. Phosphorylation of the stargazin protein at Thr321 by Protein Kinase A regulates its interaction with PSD-95 and synaptic targeting of AMPA receptors (Choi et al., 2002).
Storage
100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.
Product Specific References
Selvakumar, B., Campbell, P. W., Milovanovic, M., Park, D. J., West, A. R., Snyder, S. H., & Wolf, M. E. (2014). AMPA receptor upregulation in the nucleus accumbens shell of cocaine-sensitized rats depends upon S-nitrosylation of stargazin. Neuropharmacology, 77, 28-38. PMID: 24035918
>>> 更多资讯详情请访问蚂蚁淘商城
