Designations: Raji Depositors:  W Henle Biosafety Level: 2 [CELLS CONTAIN HERPESVIRUS (EBV) ] Shipped: frozen Medium Serum: See Propagation Growth Properties: suspension Organism: Homo sapiens Morphology: lymphoblast Source: Disease: Burkitt\'s lymphoma Cell Type: B lymphocyte; Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Isolation: Isolation date: 1963 Applications: transfection host Virus Resistance: The cells are partially resistant to poliovirus and vesicular stomatitis viruses. DNA Profile (STR): Amelogenin: X,Y CSF1PO: 10,12 D13S317: 13 D16S539: 8,11 D5S818: 10,13 D7S820: 10 THO1: 6,7 TPOX: 8,13 vWA: 16,19 Cytogenetic Analysis: Karyotype 100% stable within male diploid stemline of 46. Cells with 47 chromosomes frequently contained an extra E group chromosome.; There is 6% polyploidy and occasional disparity in the size of the homologs of the number 1 chromosome and the number 4 chromosome. Age: 11 years Gender: male Ethnicity: Black Comments: The Raji line of lymphoblast-like cells was established by R.J.V. Pulvertaft in 1963 from a Burkitt\'s lymphoma of the left maxilla of an 11-year-old Black male. EBNA positive. Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively the cells may be collected by centrifugation. Cultures can then be established by resuspending the cells in fresh medium at 4 X 10(5) viable cells/ml. A maximum of 3 X 10(6) viable cells/ml is obtainable. Medium Renewal: Every 2 to 3 days Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001recommended serum:ATCC 30-2020derivative:ATCC CCL-214 References: 22142: Pulvertaft JV. Cytology of Burkitt\'s tumour (African lymphoma). Lancet 1: 238-240, 1964. PubMed: 14086209 22169: Epstein MA, Barr YM. Characteristics and mode of growth of tissue culture strain (EB1) of human lymphoblasts from Burkitt\'s lymphoma. J. Natl. Cancer Inst. 34: 231-240, 1965. PubMed: 14293790 22550: Ohsugi Y, et al. Tumorigenicity of human malignant lymphoblasts: comparative study with unmanipulated nude mice, antilymphocyte serum-treated nude mice, and X- irradiated nude mice. J. Natl. Cancer Inst. 65: 715-718, 1980. PubMed: 6932523 22572: Moore PS, et al. Primary characterization of a herpesvirus agent associated with Kaposi\'s sarcoma. J. Virol. 70: 549-558, 1996. PubMed: 8523568 26253: Epstein MA, et al. Morphological and virological investigations on cultured Burkitt tumor lymphoblasts (strain Raji). J. Natl. Cancer Inst. 37: 547-559, 1966. PubMed: 4288580 26254: . . Trans. N.Y. Acad. Sci. 29: 61, 1966. 32395: Clark RA, et al. Tenascin supports lymphocyte rolling. J. Cell Biol. 137: 755-765, 1997. PubMed: 9151679 32448: Rich SA, et al. Purification, microresequencing, and immunolocalization of p36, a new interferon-alpha-induced protein that is associated with human lupus inclusions. J. Biol. Chem. 271: 1118-1126, 1996. PubMed: 8557639 16173680: U.S. Pharmacopeia USP Monographs: Technetium 99mTc Fanolesomab Injection. Rockville, MD: USP32-NF27, 2005

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