- Extract high quality & purity total RNA including miRNA
- No phenol step required - isolate all RNA in one fraction
- Elute extracted total RNA in small volume (20 µL)
- Bind & elute all RNA irrespective of size or GC content, without bias.
- Efficiently extract small RNA irrespective of GC content
- Very sensitive & linear down to a few cells without the need for carrier RNA
- Convenient & fast spin column format
- Isolate from a wide variety of specimens
- Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen"s NGS services
- Buffer chemistry inactivates viruses including SARS-CoV-2 - Explore the Application Note
Figure 1. Great Isolation Sensitivity. Norgen"s Total RNA Purification Micro Kit allows sensitive RNA extraction from as little as a single cell. Total RNA was extracted from a decreasing number of HeLa cells. FiveµL of the eluted RNAwas usedas the template in a 20 µLRT-qPCR reaction to detect the human S14 transcript. The S14 was detected from as little as a single cell.
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Total RNA Purification Micro Kit Figure 1
Figure 2. High Quality RNA Isolated from Laser-Captured Microdissection (LCM). Norgen"s Total RNA Purification Micro Kit allows sensitive but high quality RNA extraction from small inputs such as Laser-Captured Microdissection (LCM). Total RNA was isolated from 6 different LCM samples. RNA yield and quality (260/280 ratio) were assessed by NanoVue spectrophotometry. Norgen"s Total RNA Purification Micro Kit recovered good amounts of RNA with excellent 260/280 ratio.
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Total RNA Purification Micro Kit Figure 2
Figure 3. Better Efficiency of RNA Recovery at Single Cell Input. Norgen"s Total RNA Purification Micro Kit allows sensitive RNA extraction from as little as a single cell and outperforms competitor"s kits that claim similar sensitivity. Total RNA was extracted from a decreasing number of HeLa cells using Norgen"s Total RNA Purification Micro Kit and a competitors spin column purification kit. The extracted RNA was subjected to RT-qPCR to detect the human S15 and 5S rRNA transcript transcript in the isolated RNA. RNA isolated by Norgen"s Total RNA Purification Micro Kit showed much higher relative expression of each RNA transcript tested at all cell input number down to single cell.]">

Total RNA Purification Micro Kit Figure 3
Total RNA Purification Micro Kit
This kit extracts total RNA from low cell number samples and elutes in a convenient 20 µL elutionfor a number of downstream applications.
This kit is suitable for the isolation of total RNA from a range of samples including small input of cells, needle biopsies, LCM, CTCand other low cell number samples. Extract high quality and purity RNA with excellent RIN values and A260/A280suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.
The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
Isolate RNA after Purifying EVs and Exosomes
Ultracentrifugation, Exoquick, Filtration
| Cat. # | Name | Elution Volume | Plasma/Serum | Urine | Cell-Culture Media |
|---|
| 55000 | Plasma/Serum RNA Purification Mini Kit | 10 - 25 µL | 50 µL - 1 mL | 250 µL - 1 mL | 5 - 10 mL |
| 35300 | Total RNA Purification Micro Kit | 20 - 50 µL | 1 - 4 mL | 2 - 10 mL | 10 - 20 mL |
| 17200 | Total RNA Purification Kit | 50 - 100 µL | 4 - 10 mL | 11 - 30 mL | 20 - 35 mL |
Top 5 Reasons to Choose Silicon Carbide RNA Extraction Technology
加拿大 . Norgen Biotek
Norgen Biotek
加拿大Norgen Biotek Corp www.norgenbiotek.com
Norgen Biotek是来自加拿大的著名生物技术公司,生产基于专利技术上的核酸和蛋白质纯化及富集产品。Norgen Biotek 在2003年获得加拿大国家研究委员会颁发的科技创新奖。目前该公司的产品多达40余种,覆盖基因组和蛋白组,广泛地被用于药厂及科研院所研究和新产品开发的各个方面。
Mission statement:
To provide an innovative range of first class RNA, DNA and p rotein purification, clean-up and concentration kits; and to provide dedicated and expert support services to our cus tomers and commercial partners worldwide.
Quality Policy:
The team at Norgen Biotek Corp. is committed to providing our customers with first class sample preparation kits for RNA, DNA and protein purification, clean-up and concentration for use in research applications, and to provide dedicated and expert support services to our cus tomers and partners worldwide.
Norgen Biotek Corp will achieve this through our commitment to:
Providing high quality, first-class, cost effective products;
Continuous improvements to our quality system, our manufacturing operations, our business practices and our products;
Continuous improvements to cus tomer satisfaction by maintaining a customer feedback process to capture and analyze customer needs and feedback on product effectiveness;
Identification, resolution, correction and prevention of quality problems
Educating and training employees to accomplish quality goals
Maintaining our Quality Management System so as to conform to ISO 13485:2003, and comply with all relevant regulatory requirements.
Values:
The values that drive the activities of Norgen Biotek are:
A commitment at all levels to the continuous improvement of our Quality Management System.
A commitment to teamwork. We will benefit from the increased potential of employees working together to solve problems and generate new ideas.
A commitment to creating a positive work atmosphere, where individuals are encouraged to develop professionally and personally through continuous learning, and where performance is recognized and rewarded.
We are committed to always conduct our business and manufacturing practices in an open and ethical manner.
We are committed to always treat our cus tomers, our coworkers and our suppliers with respect.
Company Overview:
Norgen Biotek Corp. is an innovative, biotechnology company focusing principally on advancing novel, innovative sample preparation products for nucleic acid (DNA & RNA) and protein purification, clean-up and concentration. Norgen has one platform technology with many applications based on a proprietary resin that can be applied in the purification of DNA, RNA and proteins. The uniqueness and novelty of this technology has been recognized by the National Research Council through awarding Norgen the prestigious “Innovation Leader Award for 2003”. The company holds more than a dozen issued patents and several others are pending. Norgen currently manufactures over 40 products that are used by researchers working in genomics and proteomics in academia, pharma/biotech companies, and government institutions. These products are based on Norgen’s novel technology and include:
1. RNA purification kits : Norgen manufactures the leading products in the world for the purification of total RNA, microRNA, cy toplasmic and nuclear RNA, and RNA from the exfoliated cells found in urine. Norgen’s RNA kits are the only kits in the world that isolate true total RNA (including microRNA) without the use of phenol extraction steps. Simply stated, our kits are currently the Gold Standard in the RNA purification area, offering many significant competitive advantages. Due to the fact that Norgen’s RNA purification products do not involve phase separation based on the use of phenol, they can easily be adapted to isolate total RNA, including microRNA, in high throughput. In addition, Norgen is also pioneering kits for macromolecule fractionation using one column without sample splitting. Towards this goal, Norgen has developed the RNA/DNA/Protein Kit (3-in-1) for purification of all 3 macromolecules from one sample sequentially. In addition, a recent product has been developed that added microRNA fractionation to the RNA/DNA/Protein Kit, termed the All-in-One Purification Kit (4-in-1). These products are unique and novel and offer a substantial cost and time savings over sample splitting and a several-column approach. These powerful and novel tools are designed for researchers who are interested in studying the genome, proteome and transcriptome of a single sample, such as for studies of gene expression including gene silencing experiments or mRNA knock-downs and studies involving biomarker discovery.
2. ProteoSpin™ brand protein kits : For desalting, detergent and endotoxin removal, concentration, inclusion body screening, abundant serum protein depletion, urine protein concentration and on-column peptide preparation for mass spec analysis.
3. DNA kits: For plasmid isolation, PCR purification, gel extraction, eukaryotic and prokaryotic genomic DNA isolation, and front-end sample preparation for diagnostics and forensic applications (including milk bacterial DNA isolation, urine bacterial DNA isolation, saliva DNA isolation, and urine DNA isolation).
4. Molecular weight ladders : A broad range of DNA, RNA and protein ladders are available.
Company Location
Norgen is located in a new, 24,000 square foot state-of-the-art facility in the middle of Niagara region, Ontario, Canada one block from Brock University. We are only 30 minutes from Buffalo and 1 hour from Toron to. Also, Niagara Falls and the wineries of Niagara-on-the-Lake are located within 10 minutes of the facility. The building itself is on a large property adjacent to the 406 Highway in Thorold’s technology district, and the property offers much room for expansion.