- Isolate high quality total RNA from hard to extract fatty tissues
- Isolate total RNA, including microRNA (miRNA)
- No phenol or chloroform extractions
- Fast and easy processing using rapid spin-column format
- Genomic DNA removal without the use of nucleases
- Buffer chemistry inactivates viruses including SARS-CoV-2 - Explore the Application Note
Figure 1. Isolation of High Quality Brain Total RNA including microRNA without the use of Phenol. Norgen"s Fatty Tissue RNA Purification Kit isolates high quality total RNA from fatty tissues such as brain without the use of phenol. Total RNA was isolated from equal amounts of brain tissue using Norgens Fatty Tissue RNA Purification Kit and a leading competitors kit that involves a phenol:chloroform extraction step. The purified RNA was then resolved on a 1.2% formaldehyde-agarose gel. As it can be seen, Norgen not only isolated high and consistent yields of total RNA, but the RNA was also of high quality as evidenced by intactness of the major 28S and 18S rRNA. Importantly, only Norgen"s Fatty Tissue RNA Purification Kit was able to recover the small RNA fraction, including the microRNA (red-boxed).
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Fatty Tissue RNA Purification Kit Figure 1
Figure 2. High Yield of a Diversity of RNA Species. Norgen"s Fatty Tissue RNA Purification Kit effectively recovers all sizes of RNA from large mRNA to small RNA, including microRNAs. Total RNA was isolated from equal amounts of brain tissue using Norgen"s Fatty Tissue RNA Purification Kit and a leading competitors kit that involves a phenol:chloroform extraction step. The purified RNA was then used as the template in a RT-qPCR for detecting the beta-Actin gene (Top Panel) and for detecting miR-21 (Bottom Panel). In both graphs the blue lines correspond to Norgen isolated-RNA and the red lines correspond to competitor-isolated RNA. As it can be seen, Norgen"s kit isolated higher yields of microRNA, as indicated by the lower Ct values of the blue lines (Top Panel). Also, Norgen"s kit successfully isolated similar amount of large RNA to that of the competitor"s kit (Bottom Panel) indicating the full diversity of RNA species isolated.]">

Fatty Tissue RNA Purification Kit Figure 2
Fatty Tissue RNA Purification Kit
This kit was deigned to extract high quality and yield from fatty tissues like brain, breast, adipose tissues, etc. Often tissues that have high fat contents like brain, breast tissues, etc., can lead to low quality and yield RNA with no-optimized kit.
This kit provides a rapid and optimized method for the isolation and purification of total RNA from animal tissues with high lipid content, including brain and adipose tissues. The kit purifies all sizes of RNA down to microRNA (miRNA) without the use of inhibitory phenol or chloroform. While the kit is optimized for fatty tissues, it can also be used for most mammalian tissue samples.
The purified RNA is of the highest integrity, and can be used in a number of downstream applications including RT-qPCR, microarrays, NGS.
加拿大 . Norgen Biotek
Norgen Biotek
加拿大Norgen Biotek Corp www.norgenbiotek.com
Norgen Biotek是来自加拿大的著名生物技术公司,生产基于专利技术上的核酸和蛋白质纯化及富集产品。Norgen Biotek 在2003年获得加拿大国家研究委员会颁发的科技创新奖。目前该公司的产品多达40余种,覆盖基因组和蛋白组,广泛地被用于药厂及科研院所研究和新产品开发的各个方面。
Mission statement:
To provide an innovative range of first class RNA, DNA and p rotein purification, clean-up and concentration kits; and to provide dedicated and expert support services to our cus tomers and commercial partners worldwide.
Quality Policy:
The team at Norgen Biotek Corp. is committed to providing our customers with first class sample preparation kits for RNA, DNA and protein purification, clean-up and concentration for use in research applications, and to provide dedicated and expert support services to our cus tomers and partners worldwide.
Norgen Biotek Corp will achieve this through our commitment to:
Providing high quality, first-class, cost effective products;
Continuous improvements to our quality system, our manufacturing operations, our business practices and our products;
Continuous improvements to cus tomer satisfaction by maintaining a customer feedback process to capture and analyze customer needs and feedback on product effectiveness;
Identification, resolution, correction and prevention of quality problems
Educating and training employees to accomplish quality goals
Maintaining our Quality Management System so as to conform to ISO 13485:2003, and comply with all relevant regulatory requirements.
Values:
The values that drive the activities of Norgen Biotek are:
A commitment at all levels to the continuous improvement of our Quality Management System.
A commitment to teamwork. We will benefit from the increased potential of employees working together to solve problems and generate new ideas.
A commitment to creating a positive work atmosphere, where individuals are encouraged to develop professionally and personally through continuous learning, and where performance is recognized and rewarded.
We are committed to always conduct our business and manufacturing practices in an open and ethical manner.
We are committed to always treat our cus tomers, our coworkers and our suppliers with respect.
Company Overview:
Norgen Biotek Corp. is an innovative, biotechnology company focusing principally on advancing novel, innovative sample preparation products for nucleic acid (DNA & RNA) and protein purification, clean-up and concentration. Norgen has one platform technology with many applications based on a proprietary resin that can be applied in the purification of DNA, RNA and proteins. The uniqueness and novelty of this technology has been recognized by the National Research Council through awarding Norgen the prestigious “Innovation Leader Award for 2003”. The company holds more than a dozen issued patents and several others are pending. Norgen currently manufactures over 40 products that are used by researchers working in genomics and proteomics in academia, pharma/biotech companies, and government institutions. These products are based on Norgen’s novel technology and include:
1. RNA purification kits : Norgen manufactures the leading products in the world for the purification of total RNA, microRNA, cy toplasmic and nuclear RNA, and RNA from the exfoliated cells found in urine. Norgen’s RNA kits are the only kits in the world that isolate true total RNA (including microRNA) without the use of phenol extraction steps. Simply stated, our kits are currently the Gold Standard in the RNA purification area, offering many significant competitive advantages. Due to the fact that Norgen’s RNA purification products do not involve phase separation based on the use of phenol, they can easily be adapted to isolate total RNA, including microRNA, in high throughput. In addition, Norgen is also pioneering kits for macromolecule fractionation using one column without sample splitting. Towards this goal, Norgen has developed the RNA/DNA/Protein Kit (3-in-1) for purification of all 3 macromolecules from one sample sequentially. In addition, a recent product has been developed that added microRNA fractionation to the RNA/DNA/Protein Kit, termed the All-in-One Purification Kit (4-in-1). These products are unique and novel and offer a substantial cost and time savings over sample splitting and a several-column approach. These powerful and novel tools are designed for researchers who are interested in studying the genome, proteome and transcriptome of a single sample, such as for studies of gene expression including gene silencing experiments or mRNA knock-downs and studies involving biomarker discovery.
2. ProteoSpin™ brand protein kits : For desalting, detergent and endotoxin removal, concentration, inclusion body screening, abundant serum protein depletion, urine protein concentration and on-column peptide preparation for mass spec analysis.
3. DNA kits: For plasmid isolation, PCR purification, gel extraction, eukaryotic and prokaryotic genomic DNA isolation, and front-end sample preparation for diagnostics and forensic applications (including milk bacterial DNA isolation, urine bacterial DNA isolation, saliva DNA isolation, and urine DNA isolation).
4. Molecular weight ladders : A broad range of DNA, RNA and protein ladders are available.
Company Location
Norgen is located in a new, 24,000 square foot state-of-the-art facility in the middle of Niagara region, Ontario, Canada one block from Brock University. We are only 30 minutes from Buffalo and 1 hour from Toron to. Also, Niagara Falls and the wineries of Niagara-on-the-Lake are located within 10 minutes of the facility. The building itself is on a large property adjacent to the 406 Highway in Thorold’s technology district, and the property offers much room for expansion.