With its quantum yield approaching unity, ROX has become a popular dye for qPCR and microscopy. This bright fluorophore is available as an alkyne for copper-catalyzed Click chemistry reaction.

This is a pure 5-isomer of ROX.

ROX is prone to oxidation. Therefore, its derivatives should be preferably stored in inert atmosphere.

General properties

Appearance:	purple solid
Mass spec M+ increment:	571.3
Molecular weight:	571.66
Molecular formula:	C36H33N3O4
Solubility:	good in DMSO, DMF, methanol, ethanol
Quality control:	NMR 1H, HPLC-MS (95%)
Storage conditions:	Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation maximum, nm:	570
ε, L⋅mol−1⋅cm−1:	80000
Emission maximum, nm:	591
Fluorescence quantum yield:	1.00

Lumiprobe, LLC.是美国一家高品质生物技术公司，专业提供分子生物学研究用的活性荧光染料。主要产品有：活性染料（Reactive Dye）和SYBR Green I 染料，定制合成荧光双标记引物。产品主要应用：点击化学（Click Chemistry）、蛋白质组学研究中的双向荧光差异凝胶电泳（2D DIGE）和实时荧光定量PCR（Realtime PCR）。 Since 2006, we manufacture and sell advanced chemicals for life science research, and diagnostics. Our catalog includes fluorescent dyes, phosphoramidites for oligonucleotide synthesis, Click chemicals, and other reagents. Most of the items are kept in stock, and ready for immediate shipping to worldwide locations. 产品列表： No. 品牌 货号 名称 规格 1 Lumiprobe 11030 Cy3 azide   100 uL 2 Lumiprobe 12030 Cy3.5 azide 100 uL 3 Lumiprobe 13030 Cy5 azide 100 uL 4 Lumiprobe 14030 Cy5.5 azide 100 uL 5 Lumiprobe 14130 FAM azide, 5- isomer, 100 uL 6 Lumiprobe 15130 FAM azide, 6- isomer 100 uL 7 Lumiprobe 14230 R110 azide, 5- isomer 100 uL 8 Lumiprobe 12230 ROX azide, 6- isomer 100 uL 9 Lumiprobe 17130 TAMRA azide, 5- isomer 100 uL 10 Lumiprobe 33720 Azidobutyric acid NHS ester 10 mg 11 Lumiprobe 30720 Pentynoic acid STP ester 5 mg 12 Lumiprobe 42260 Alkyne Amidite, 5'-terminal 1 g 13 Lumiprobe 21360 Alkyne Amidite, hydroxyprolinol 250 mg 14 Lumiprobe 11320 Sulfo-Cy3 NHS ester 1 mg 15 Lumiprobe 11020 Cy3 NHS ester 1 mg