Details:
Tested Applications	ELISA, Western Blot
Target Information	Sir1 is a 76.9kDa, S. cerevisiaetranscription repressor, member of the Sir(Silent Information Regulator) family
Product Format	Affinity Purified
Alternate Names	Heterochromatin protein SIR1, Silent information regulator 1
Clone	1C12B12
Host	Mouse
Isotype	IgG1
Immunogen	Recombinant Protein
Epitope	Unknown
Relevance	Involved in the establishment, but not the maintenance, of heterochromatic silencing at the cryptic mating-type loci HMR and HML. Is recruited by interacting with the ORC1 subunit of the origin recognition complex (ORC), which binds to HML-I or HMR-E silencers, DNA elements that direct the formation of silent chromatin at the mating-type loci. Establishes transcriptional silencing by recruiting the three other SIR proteins, SIR2, SIR3, and SIR4, that function directly in silenced chromatin and establish repression. Also found in centromeric chromatin. Binds to and helps retain CAC1, a subunit of chromatin assembly factor I (CAF-I) at centromeric loci independent on the other SIR proteins.
Specificity	Yeast (Saccharomyces cerevisiae)
Reported Reactivity	Unknown
Reported Applications	Unknown

What's Happening at NeoClone Biotechnology You're probably wondering, "Now that BioLegend owns all your former catalog products, what the heck are you doing up there in Madison?" I'm glad you asked. We're still making great monoclonal antibodies, of course! NeoClone has been making custom murine antibodies via hybridoma technology for years. In fact, that's where our catalog came from in the first place! We decided that we needed to return our focus to custom projects, especially the really difficult targets that researchers had been bringing us all along. You know, the targets that other companies couldn't make due to problems with tolerance or toxicity or bizarre secondary structures. Then we decided to branch out into other methods of generating antibodies. We brought on new staff with years of experience in Antibody Phage Display and Camelid VHH antibodies. We've been upping our game in the Bioinformatics arena as well, giving us an edge in determining the best way to make YOUR antibody. Unlike other antibody development companies, we're not tied to one particular method. In the world of antibody development, one size does NOT fit all. So, you tell us what you want. We'll make sure we get it for you.