NanoFuel®FLASHAssayfor GaussiaLuciferase

TheNanoFuel®FLASHAssaykitprovidesenoughbufferforapprox.1000assaysandperformsbestwithaluminometerequippedwithaninjectionport.Forassaysrequiringalongerlastingluminescence(e.g.high-throughputscreenings)orifsensitivityisnotanissuewerecommendour NanoFuelGLOWAssaykit(Cat.#320).

References:GaussialuciferaseisusedasareporterinconjunctionwithCRISPRtoinvestigatethefunctionofnoncodingRNAs(ncRNA) Namkung,etal.

50ml	Lysisbuffer-optimizedformildlysisandminimal GLucactivity reduction
50ml	GaussiaDilutionBuffer-stABIlizesGLucandprovides optimalreactionconditions
50ml	CoelenterazineDilutionBuffer-stabilizesCTZ in aequeoussolution
1ml	50xCoelenterazine(CTZ)substrate containing>95%pureCTZ

 

 

 

  AdvantagesoftheFLASHAssayReagentforGaussiaLuciferase:

• createsoptimalconditionsforGLucandmutatedGLucversions
• lowbackground,thushigherS/Nratio
• higheroverallsignal
• bufferhasproteinstabilizingproperties

CompleteManual     QuickInstructions

Shipping:viaFedExorUPSovernightdeliveryfordomestic,allow2-4daysforInternationaldelivery.Storeat-80°Corindividualcomponentsasdirecteduponarrival.

Coldpacksrequired.Additionalshippingcostsapply.

NanoLight 纳米光®科技公司是一家生物技术公司，开发天然的、发光的海洋蛋白质或海洋生物发光的广泛应用。纳米光部门致力于成为制药、农业和研究产品行业中高价值试剂和报告系统的领导者。 NanoLight® Technology is the technology division of Prolume® Ltd., a company focusing upon broad based applications of marine bioluminescence. The NanoLight® division is dedicated to providing scientists with the highest quality reagents, luciferins (NanoFuel®s), recombinant bioluminescent proteins luciferases (NanoLight®s), photoproteins (NanoFlashTM) and fluorescent proteins (NanoFluorsTM). 品牌 货号 名称 规格 NanoLight 3031 Coelenterazine 500 ug CTZ / vial NanoLight 3032 Coelenterazine 500 ug CTZ / vial NanoLight 3011 h-Coelenterazine 500 ug CTZ / vial