Description:RNAse Inhibitor is an acidic, 52 kDa protein that is a potent, non-competitive inhibitor of pancreatic-type ribonucleases such as RNase A, RNase B, and RNase C. The enzyme is provided as a fusion of the porcine RNAse Inhibitor gene with a proprietary, 22.5 kDa protein tag.Application:Inhibits ribonucleases (RNases) A, B and CSource:A recombinant E. coli strain carrying the porcine RNAse Inhibitor gene.Supplied in:20 mM Hepes-KOH50 mM KCl8 mM DTT50% GlycerolpH 7.5 @ 25°CUnit Definition:One unit is defined as the amount of enzyme required to inhibit 50% the hydrolysis of cytidine 2\',3\'-cyclic monophosphate by 5 ng of RNAse A.(1)Recommended Storage Condition: -20°CReference:1. Blackburn, P., 1979. Ribonulcease Inhibitor from Human Placenta: Rapid Purification and Assay. The Journal of Biological Chemistry, Vol. 254, No. 24 pp 12484-12487. |
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