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蚂蚁淘在线 / 品牌中心 / Kapa biosystems / Kapa-biosystems/Kapa-Stranded mRNA-Seq试剂盒/KK8400/24文库

Kapa-biosystems/Kapa-Stranded mRNA-Seq试剂盒/KK8400/24文库

价格
￥18720.00
货号：KK8400
浏览量：127
品牌：Kapa biosystems
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商品描述

Description:

StrandedRNA-SeqKit

KAPAStrandedmRNA-SeqKits

Evendifficultmessagesshouldbeunderstood.

KAPAStrandedmRNA-SeqKitsincludealltheenzymesandbuffersrequiredforCDNAlibrarypreparationforIlluminaNext-GenerationSequencing,utilizing100ng–4µgoftotalRNA.KAPAmRNACaptureBeadsareincludedforisolationofpoly(A)-tailedRNA.Kitsprovideprecisemeasurementofstrandorientation(>99%),uniformcoverage,andhigh-confidencemappingofalternatetranscripts,andareoptimizedfortheimprovedcoverageofGC-richandlow-abundancetranscripts.*KitscontainKAPAHiFiforhigh-efficiencyandlow-biaslibraryamplification,aswellasKAPAmRNACaptureBeadsandastreamlined,“with-bead”protocol.

KAPAStrandedRNA-SeqKitsincludealltheenzymesandbuffersrequiredforcDNAlibrarypreparationforIlluminaNext-GenerationSequencing,butdonotcontaintheKAPAmRNACaptureBeads.Kitscanbeusedtopreparelibrariesfrom10–400ngofeitherpoly(A)-selected,ribosomallydepleted,ortotalRNA.

NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.

Download AdapterandBeadCalculator

 

*Dataonfile.
ForResearchUseOnly.Notforuseindiagnosticprocedures.

ProductHighlights

Improved coverage of GC-rich transcripts. The 5

Uncoverchallengingtranscripts 

  • ImprovedcoverageofGC-richtranscripts
  • Enhancedidentificationofexonicregions
Improved coverage of low-abundance transcripts. GLTPD1, a lesser-expressed transcript, is covered more comprehensively with the KAPA Stranded mRNA-Seq Kit (green) at 500 ng input total RNA (outlined in red). In comparison, Illumina TruSeq Stranded mRNA Sample Prep Kit (orange) shows coverage gaps, even with higher inputs (4 μg). Data on file.

Detectlow-abundancetranscripts

  • Enablesidentificationoftranscriptsmissedbycompetitorkits,evenwithhighinput*
  • Highuniformityacrossvaryingamountsofsampleinput*
30M),KAPAStrandedmRNA-SeqlibrariesgenerateagreaterpercentageofmappedreadsandlowerduplicationratesthananalogouslibrariespreparedwiththeIlluminaStrandedmRNASamplePrepKitwhilemaintaining99%strandspecificity.Dataonfile.">Highly mapped reads and strand specificity enable sensitive detection of expressed genes. With similar numbers of filter-passed reads (>30M),KAPAStrandedmRNA-SeqlibrariesgenerateagreaterpercentageofmappedreadsandlowerduplicationratesthananalogouslibrariespreparedwiththeIlluminaStrandedmRNASamplePrepKitwhilemaintaining99%strandspecificity.Dataonfile.

Identifymoregenes

  • HigherpercentageofuniquelymappedreadscomparedtoIllumina TruSeq™StrandedmRNASamplePrepKits*
  • Lowerduplicationratesyieldbettercoverage
Minimal positional bias. With intact, high-quality RNA input, 3

Maintainhighcoverageuniformity

  • Minimal5’–3′biasacrosstranscripts
  • Moreuniformdistributionofreadsovereachtranscript

 

*Dataonfile.

RelatedProducts

SequencingchallengingRNAsamples?HowaboutDNA?CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

Sample QC

KAPAhgDNAQuantificationandQCKit

Library Amplification

KAPALibraryAmplificationKits

Library Preparation

KAPAHyperPrepKits

Library Quantification

KAPALibraryQuantificationKits

Applications:

Applications
  • Geneexpression
  • Singlenucleotidevariation(SNV)discovery
  • Post-transcriptionalSNVs
  • Fusiongeneidentification
  • Targetedtranscriptome
  • Wholetranscriptome

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

Kitscanbestoredforupto8 months at-20˚C. KAPAmRNACaptureBeadscanbestoredforupto8months at-4˚C.

Components

mRNA_Component Chart

Specifications

Spec
Description
CompatIBLePlatform
IlluminaHiSeq,NextSeq,MiSeqandGAIIx
StartingMaterial
TotalRNA,mRNA,orribosomaldepletedRNA
InputAmount
StrandedmRNA-SeqKits:100ng–4µgStrandedRNA-SeqKits:10ng–400ng
Kapa是世界上著名的生物技术公司。总部位于美国波士顿, 公司的主要理念是运用分子和遗传学技术来打造新一代的生物科学和医学诊断产品。Kapa公司目前主要是在“直接进化（directed evolution technology platform）”的技术平台上来筛选和生产高品质的生物酶。目前生物医药领域所使用的大部分生物酶都只是天然存在形式，即使有些酶经过基因工程改造，但是由于酶分子自身的结构和功能限制，要想得到真正满足科研工作人员需要的产品，却是一件费时费力的事情，而且常常达不到预期的要求。“直接进化”技术正是针对这一现实情况，运用遗传，基因工程，生物信息学等技术来在分子水平上设计真正满足不同实际要求的生物酶。通过创新，Kapa为广大的科研人员提供了一系列拥有多项技术的产品，不仅能够满足普通科研需要，同时为高通量，基因芯片等高新技术的普及创造了条件。 运用“直接进化”技术，Kapa目前已经开发