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蚂蚁淘在线 / 品牌中心 / Kapa biosystems / 用于Illumina/KK8001/30 mL的Kapa生物系统/文库制备

用于Illumina/KK8001/30 mL的Kapa生物系统/文库制备

价格
￥9000.00
货号：KK8001
浏览量：127
品牌：Kapa biosystems
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商品描述

Description:

KAPAPureBeads(30mL)

LibraryPreparationforIllumina

It’scomplex,butwehaveitcovered.

KAPADNALibraryPreparationKitsforIllumina®sequencingplatformscontainevolvedandoptimallyformulatedenzymesthatenablethehighestoverallcoveragefromtheleastamountoftotalsequencing.

KitsareoptimizedtoachievesignificantlyhigherlibraryyieldsandcontainKAPAHiFiforhigh-fidelity,high-efficiencyandlow-biaslibraryamplification.Thisensuresthehighestlibraryandsequencedataquality,particularlyfromlow-inputanddifficultsamplessuchasFFPEandChIPDNA.

NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.

DownloadourKAPA AdapterandBeadCalculator

 

ForResearchUseOnly.Notforuseindiagnosticprocedures.

ProductHighlights

Key sequencing metrics (library diversity, left and duplication rates, right) for libraries prepared for exome capture (SureSelect All Human Exome V5 + UTR, Agilent Technologies) from different amounts of Covaris-sheared DNA using the KAPA Library Preparation Kit (green) or a competitor kit (blue). Optimally formulated and evolved enzymes, combined with a highly optimized “with-bead” protocol result in higher library yields with the KAPA Library Preparation Kit. This translates to higher library diversity and lower duplication rates, even with significantly less input DNA. Paired-end sequencing (2 x 100 bp) was performed on an Illumina HiSeq 2000. Data courtesy of the Translational Genomics Research Institute. Data on file.
Key sequencing metrics (library diversity, left and duplication rates, right) for libraries prepared for exome capture (SureSelect All Human Exome V5 + UTR, Agilent Technologies) from different amounts of Covaris-sheared DNA using the KAPA Library Preparation Kit (green) or a competitor kit (blue). Optimally formulated and evolved enzymes, combined with a highly optimized “with-bead” protocol result in higher library yields with the KAPA Library Preparation Kit. This translates to higher library diversity and lower duplication rates, even with significantly less input DNA. Paired-end sequencing (2 x 100 bp) was performed on an Illumina HiSeq 2000. Data courtesy of the Translational Genomics Research Institute. Data on file.
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Achievegreatermolecularcomplexity

  • Higheryieldsofadapter-ligatedlibrarytranslatestohighermolecularcomplexity
  • Fewercyclesofamplificationareneeded,whichresultsinlowerPCRduplicationrates
  • PCR-freeworkflowspossIBLefrominputs≥100ng*
Indexed libraries were constructed and amplified from 100 ng human genomic DNA using either the KAPA Library Preparation Kit (right) or the standard library preparation reagents and protocol in use at The Broad Institute (left). Individual libraries were quantified using qPCR, pooled prior to denaturation, and sequenced (pair-ended, 2 x 25 bp) on an Illumina HiSeq 2000. Libraries prepared using the KAPA Library Preparation Kit resulted in more uniform coverage distribution across the range of GC-content. Data courtesy of The Broad Institute. Data on file.
Indexed libraries were constructed and amplified from 100 ng human genomic DNA using either the KAPA Library Preparation Kit (right) or the standard library preparation reagents and protocol in use at The Broad Institute (left). Individual libraries were quantified using qPCR, pooled prior to denaturation, and sequenced (pair-ended, 2 x 25 bp) on an Illumina HiSeq 2000. Libraries prepared using the KAPA Library Preparation Kit resulted in more uniform coverage distribution across the range of GC-content. Data courtesy of The Broad Institute. Data on file.
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Reduceamplificationbiasandimprovecoverage

  • KAPAHiFireducesPCRbias*
  • ImprovescoverageuniformityofGC-andAT-richregions,promoters,lowcomplexityandotherchallengingregions*
Key sequencing metrics (fraction of duplicates, left and post-capture library complexity, right) for FFPE libraries prepared with the KAPA Library Preparation Kit for target capture (SeqCap EZ custom panel, 279 genes, 1 MB; Roche Nimblegen). Of each of the eight colon/gastric cancer FFPE samples, 250 ng FFPE DNA was Covaris-sheared for automated library construction, using a Biomek FXp instrument (Beckman Coulter). KAPA HiFi HotStart ReadyMix was used for both pre- and post-capture amplification (8 and 12 cycles, respectively). Paired-end sequencing (2 x 75 bp) was performed on an Illumina MiSeq. Data courtesy of Memorial Sloane-Kettering Cancer Center. Data on file.
Key sequencing metrics (fraction of duplicates, left and post-capture library complexity, right) for FFPE libraries prepared with the KAPA Library Preparation Kit for target capture (SeqCap EZ custom panel, 279 genes, 1 MB; Roche Nimblegen). Of each of the eight colon/gastric cancer FFPE samples, 250 ng FFPE DNA was Covaris-sheared for automated library construction, using a Biomek FXp instrument (Beckman Coulter). KAPA HiFi HotStart ReadyMix was used for both pre- and post-capture amplification (8 and 12 cycles, respectively). Paired-end sequencing (2 x 75 bp) was performed on an Illumina MiSeq. Data courtesy of Memorial Sloane-Kettering Cancer Center. Data on file.
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Createhigh-qualitylibrariesfromchallengingsamples

  • High-qualitywholeexomesequencingfromlibrariespreparedusingaslittleas10nghumangenomicDNA*
  • Highsuccessrateswith250ngFFPEorless*
  • Routinelibraryconstructionfrom≥100pgChIPDNA*
KAPA Library Quantification methods (96- and 384-well) format are available for the Sciclone NGS and NGSx workstation (PerkinElmer) and other instruments used in high-throughput NGS sample preparation pipelines.

Automatewithpre-validatedscripts

 

*Dataonfile.

Applications:

Applications
  • WholeGenomeSequencing
  • ChIPSequencing
  • WholeExomeSequencing
  • AmpliconSequencing
  • TargetedSequencing(Capture)
  • MethylSequencing

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

Kitscanbestoredforupto18months at-20˚C.

Kitsincludereagentsforendrepair,A-tailing,ligationandlibraryamplification.PCR-freeversions(withoutlibraryamplificationreagent)arealsoavailable.“With-bead”kitsincludePEG/NaClsolutionforSPRIbeadcleanups.
All96-librarykitsareautomationfriendly.

Components

Specifications

Spec
Description
CompatiblePlatform
IlluminaHiSeq,MiSeq,NextSeqandGAIIx
LibraryType
DNA
StartingMaterial
FragmentedDNAorPCRamplicons
InputAmount
100pg–5µg
SequencingApplications
WholeGenomeSequencingWholeExomeSequencingTargetedSequencing(custompanels)ChIP-SeqAmpliconSequencingMethyl-Seq
Kapa是世界上著名的生物技术公司。总部位于美国波士顿, 公司的主要理念是运用分子和遗传学技术来打造新一代的生物科学和医学诊断产品。Kapa公司目前主要是在“直接进化（directed evolution technology platform）”的技术平台上来筛选和生产高品质的生物酶。目前生物医药领域所使用的大部分生物酶都只是天然存在形式，即使有些酶经过基因工程改造，但是由于酶分子自身的结构和功能限制，要想得到真正满足科研工作人员需要的产品，却是一件费时费力的事情，而且常常达不到预期的要求。“直接进化”技术正是针对这一现实情况，运用遗传，基因工程，生物信息学等技术来在分子水平上设计真正满足不同实际要求的生物酶。通过创新，Kapa为广大的科研人员提供了一系列拥有多项技术的产品，不仅能够满足普通科研需要，同时为高通量，基因芯片等高新技术的普及创造了条件。 运用“直接进化”技术，Kapa目前已经开发