Agrisera/GSH (Glutathione) ELISA kit/AS16 4095/

作者: 时间:2026-04-18 点击量:

Background | This ELISA kit uses Competitive-ELISA as the method. The provided microtiter plate is pre-coated with GSH. During the reaction, GSH in the sample or standard competes with a fixed amount of GSH on the solid phase supporter for sites on the anti-GSH biotinylated detection antibody. Excess conjugate and unbound sample or standard are washed away from the plate, and a secondary HRP-streptavidin (SABC) antibody is added to each microplate well and incubated. To develope the enzyme-substrate reaction a TMB substrate solution is added to each well and addition of a sulphuric acid solution terminates this reaction. Color change is measured spectrophotometrically at 450 nm wavelength. The concentration of GSH in the samples can be determined from the standard curve.

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