CA-C149

Name

Elite^TM Caspase 9 Assay Kit (Fluorescence)

Description

Caspase 9 is proven to have selectivity for the peptide sequence Leu-Glu-His-Asp (LEHD). This kit uses (Ac-LEHD)₂-R110 as a fluorogenic indicator for caspase 9 activity. Cleavage of R110 peptides by caspase 9 generates strong fluorescent rhodamine 110 (R110) that is monitored at the emission between 520 nm and 530nm with the excitation between 480 nm and 500 nm.

Application

The Elite^TM Caspase 9 Assay Kit provides all the essential components. The assay is robust and can be readily adapted for high throughput screening (HTS). It can be used to either quantify the activated caspase 9 activities in apoptotic cells or screen the caspase 9 inhibitors. Many labs have used this kit for high throughput screening.

Size

2 plates (96-well)

Detection

Fluorescence microplate reader